Rational Rubber Extraction and Simultaneous Determination of Rubber Content and Molecular Weight Distribution in Taraxacum kok-saghyz Rodin by Size-Exclusion Chromatography
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Taraxacum kok-saghyz Rodin (TKS) is a potential nature rubber source crop. To extract rubber from TKS effectively and quantitate the TKS rubber accurately, we developed a method that can simultaneously determine the rubber content and molecular weight distribution (MWD) of TKS by size-exclusion chromatography (SEC). The solvent types and rubber extraction schemes for sample pretreatment were discussed. The results indicated that the indirect ultrasonic method of extracting rubber from TKS roots required much shorter extraction time than conventional extraction method, and the scheme did not affect the characterization of MWD. In addition, SEC can be used for TKS rubber quantification with a limit of detection of 0.03 mg mL−1, and the intra-day and inter-day precision were 2.31% and 5.24%, respectively. The MWD of TKS rubber demonstrated a unimodal distribution. This developed method has been applied to the determination of real-life samples successfully.
KeywordsTaraxacum kok-saghyz Rodin Rubber content Size-exclusion chromatography Quantitative analysis Molecular weight distribution
The authors acknowledge the support of the National Key Research and Development Program of China (2016YFF0203703), the NSFC Grant (51673012), and the Fundamental Research Funds for the Central Universities (PYBZ1828).
This study was funded by the National Key Research and Development Program of China (2016YFF0203703), the NSFC Grant (51673012), and the Fundamental Research Funds for the Central Universities (PYBZ1828).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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