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Glycol Diglycidyl Ether and γ-Aminobutyric Acid Functionalized Silica as a Mixed Mode Stationary Phase for Capillary Liquid Chromatography

  • Shuangli Zhao
  • Feifei Luo
  • Yan Zhang
  • Yi Zhang
  • Pingang He
  • Qingjiang WangEmail author
Original
  • 11 Downloads

Abstract

In this study, a mixed mode stationary phase for capillary liquid chromatography (cLC) was prepared by chemical modification of glycol diglycidyl ether (EGDE) and γ-aminobutyric acid (GABA) bonded to the silica, named as Sil–EGDE–GABA. The Sil–EGDE–GABA was characterized by Fourier transform infrared spectroscopy and elemental analysis. The retention of nucleotide bases at different acetonitrile levels in the mobile phase indicated that the stationary phase possessed both hydrophilic chromatography (HILIC) and reversed-phase chromatography (RPLC) characteristics. Positional isomers, aniline compounds and a mixture of polar–nonpolar analytes were separated in RPLC mode. The stationary phase exhibited different separation mechanisms in the separation of positional isomers, compared with a commercial ODS column. Sulfonamides and biogenic amines were successfully separated in HILIC mode. These results demonstrated the application possibilities of the prepared Sil–EGDE–GABA as packing materials in cLC.

Keywords

Capillary liquid chromatography Sil–EGDE–GABA stationary phase Hydrophilic chromatography Reversed-phase chromatography 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 21575042).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants performed by any of the authors.

Supplementary material

10337_2019_3687_MOESM1_ESM.docx (412 kb)
Supplementary material 1 (DOCX 411 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Chemistry and Molecular EngineeringEast China Normal UniversityShanghaiPeople’s Republic of China

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