, Volume 82, Issue 4, pp 749–755 | Cite as

Retention Modeling of Gradient Elutions: Application of Iso-to-Grad Approach for LC Systems with Dual-Species Eluent

  • Vladimir Stankov
  • Matija Cvetnić
  • Mirjana Novak Stankov
  • Marko Rogošić
  • Tomislav Bolanča
  • Šime UkićEmail author
Short Communication
Part of the following topical collections:
  1. 24th International Symposium on Separation Sciences combined with 21st International Conference Analytical Methods and Human Health


Development of new chromatographic method is often time-consuming and may be shortened by mathematical modeling. Unfortunately, many of the models are limited for predefined elution programs only. The integral model uses iso-to-grad approach to transfer information from isocratic to gradient elution without such limitations, which was successfully tested for IC and HPLC using single-species eluents. Here its applicability was tested on systems using more complex eluents. An HPLC analysis of emerging contaminants using methanol/acetonitrile/water eluent was performed. Four different integral models were developed and validated. The models showed good predictive ability.


Iso-to-grad information transfer Liquid chromatography Method development Dual-species eluent 



This study was funded by Croatian Science Foundation through project entitled Modeling of Environmental Aspects of Advanced Water Treatment for Degradation of Priority Pollutants (MEAoWT) (IP-09-2014-7992).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Sample Control d.o.o.ZagrebCroatia
  2. 2.Faculty of Chemical Engineering and TechnologyUniversity of ZagrebZagrebCroatia

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