Development of Graphene Oxide Functionalized Cotton Fiber Based Solid Phase Extraction Combined with Liquid Chromatography-Fluorescence Detection for Determination of Trace Auxins in Plant Samples
- 18 Downloads
In this work, a convenient and sensitive solid phase extraction (SPE) method was developed based on graphene oxide functionalized cotton fiber (GO-CF), a SPE sorbent followed by liquid chromatography-fluorescence detection (LC-FLD) for determination of trace indole acetic acid, indole propionic acid, indole butyric acid and naphthalene acetic acid. GO-CF was synthesized via covalent bonding method on the surface of cotton fiber, and exhibited higher extraction capacity in comparison with that of cotton fiber. The conditions affecting the extraction efficiency, including sample pH, elution solvent, elution volume, extraction time, and sample volume, were optimized to obtain high extraction recoveries. Under optimal conditions, the calibration curves were linear over the concentration ranging from 2 to 200 ng mL−1 with correlation coefficients between 0.9987 and 0.9998 for all the analytes. The limits of detection were lower than 0.371 ng mL−1 for the four auxins. The intra- and inter-day relative standard deviations (RSDs) were less than 3.5 and 7.1%, respectively. The mean recoveries ranged from 73.5 to 105.4% with RSDs lower than 12.6%. The developed GO-CF–SPE–LC-FLD method has been successfully applied to the analysis of four auxins in real plant samples, including mung bean stalk, mung bean sprout, and tobacco leaf samples.
KeywordsGraphene oxide Cotton fiber Sample preparation Solid phase extraction Auxin
This study was funded by the National Natural Science Foundation of China (Grant nos. 21465008, 21265004, and 21665006), the Natural Science Foundation from Guangxi Zhuang Autonomous Region (nos. 2015GXNSFAA139024 and 2015GXNSFFA139005), the Project of High Level Innovation Team and Outstanding Scholar in Guangxi Colleges and Universities (Guijiaoren49), and the Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection.
Compliance with Ethical Standards
Conflict of interest
The authors declare no conflict of interest.
This article does not contain any studies with human participants performed by any of the authors.
Informed consent was not applicable.
- 7.Yang L, Chen Y, Zhao S, Zhang W, Du H, Deng Z, Zhang S (2016) Simultaneous determination of indole-3-acetic acid and indole-3-butyric acid in plant by field-amplified sample stacking open-tubular capillary electrochromatography based on solid-phase extraction with calixarene sorbent. Chromatographia 79:243–254CrossRefGoogle Scholar
- 10.Zhang Y, Li Y, Hu Y, Li G, Chen Y (2010) Preparation of magnetic indole-3-acetic acid imprinted polymer beads with 4-vinylpyridine and β-cyclodextrin as binary monomer via microwave heating initiated polymerization and their application to trace analysis of auxins in plant tissues. J Chromatogr A 1217:7337–7344CrossRefGoogle Scholar
- 27.Ma Z, Ge L, Lee ASY, Yong JWH, Tana SN, Ong ES (2008) Simultaneous analysis of different classes of phytohormones in coconut (Cocos nucifera L.) water using high-performance liquid chromatography and liquid chromatography–tandem mass spectrometry after solid-phase extraction. Anal Chim Acta 610:274–281CrossRefGoogle Scholar
- 28.Kojima M, Kamada-Nobusada T, Komatsu H, Takei K, Kuroha T, Mizutani M, Ashikari M, Ueguchi-Tanaka M, Matsuoka M, Suzuki K, Sakakibara H (2009) Highly sensitive and high-throughput analysis of plant hormones using MS-probe modification and liquid chromatography–tandem mass spectrometry: an application for hormone profiling in Oryza sativa. Plant Cell Physiol 50:1201–1214CrossRefGoogle Scholar
- 30.Zhong Q, Qiu X, Lin C, Shen L, Huo Y, Zhan S, Yao J, Huang T, Kawano S, Hashi Y, Xiao L, Zhou T (2014) An automatic versatile system integrating solid-phase extraction with ultra-high performance liquid chromatography–tandem mass spectrometry using a dual-dilution strategy for direct analysis of auxins in plant extracts. J Chromatogr A 1359:131–139CrossRefGoogle Scholar