Realization of 19F MRI oximetry method using perfluorodecalin
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To identify the technical aspects of the potential use of clinically approved perfluorodecalin (PFD, C10F18) for 19F magnetic resonance imaging (MRI) oximetry method at high magnetic field 7.05 T.
Materials and methods
19F T1 measurements were made on a set of PFD samples with different oxygen contents (0%, 21%, and 100%) at room (21 °C) and body temperature (37 °C). In vivo MRI studies were carried out on one healthy rat and two rats with C6 brain glioma.
The selective excitation of the magnetically equivalent 19F nuclei of CF2 groups of trans-isomer of PFD, which give a doublet at a frequency of about − 140 ppm (in relation the chemical shift of trifluoroacetic acid, which is − 76.55 ppm) should be done for correct implementation of 19F MRI oximetry method. The amount of PFD equal to 30 μl is the optimal for obtaining reliable data on the measured T1 values. In this case, the standard deviation of T1 does not exceed 5%. In vivo MRI studies showed that the values of the partial pressure of oxygen (pO2) decrease from normal values of about 38 mmHg (healthy brain) to almost 0 mmHg at the last stage of tumor growth.
The study showed the feasibility of the successful application of PFD for 19F MRI oximetry method.
KeywordsPerfluorodecalin 19F MRI oximetry method C6 rat brain glioma RARE-VTR
This work was carried out at the Moscow State University on the equipment of the Collective Using Center “Biospectrotomography” and supported by Russian Foundation for Basic Research Grant no. 17-02-00465
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All procedures were conducted in accordance with the European Community Council directives 86/609/EEC and the study was approved by the local institutional animal ethics committee.
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