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Preclinical 19F MRI cell tracking at 3 Tesla

  • Ashley V. MakelaEmail author
  • Paula J. Foster
Research Article

Abstract

Purpose

To develop methods for fluorine-19 (19F) MRI cell tracking in mice on a 3 Tesla clinical scanner. Compared to iron-based cell tracking, 19F MRI has lower sensitivity and, consequently, preclinical 19F cell tracking has only been performed at relatively high magnetic field strengths (> 3 T). Here, we focus on using 19F MRI to detect macrophages in tumors; macrophage density is an indication of tumor aggressiveness and, therefore, 19F MRI could be used as an imaging biomarker.

Methods

Perfluorocarbon (PFC)-labeled macrophages were imaged at 3 T and NMR spectroscopy was performed to validate 19F spin quantification. In vivo 19F MRI was performed on tumor-bearing mice, post-PFC at both 9.4 T and 3 T. 3 T MRI utilized varying NEX and 19F images were analyzed two different ways for 19F quantification.

Results

As few as 25,000 cells could be detected as cell pellets at 3 T. 19F quantification in cell pellets by 3 T MRI agreed with NMR spectroscopy. 19F signal was observed in the liver, spleen and tumor in all mice at 9.4 T and 3 T and there was no significant difference in 19F spin quantification.

Conclusion

This study demonstrates the ability to detect and quantify 19F signal in murine tumors using 19F MRI at 3 T.

Keywords

19-Fluorine (19F) Magnetic resonance imaging (MRI) Cancer Cell tracking Tumor-associated macrophage (TAM) 

Notes

Acknowledgements

We acknowledge the following sources of funding for AVM: Natural Sciences and Engineering Research Council, Molecular Imaging Graduate Program (Western University), Translational Breast Cancer Research Unit, Cancer Research and Technology Transfer Program and Canadian Cancer Society.

Author contributions

AVM study conception and design, acquisition of data, analysis and interpretation of data, drafting of manuscript and critical revision. PJF study conception and design, drafting of manuscript and critical revision.

Funding

This study was funded by: Canadian Institute for Health Research.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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Copyright information

© European Society for Magnetic Resonance in Medicine and Biology (ESMRMB) 2018

Authors and Affiliations

  1. 1.Robarts Research InstituteLondonCanada
  2. 2.The Department of Medical BiophysicsWestern UniversityLondonCanada

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