Abstract
Objective
To investigate the reversal effect of CQ11, a chloroquine derivative, on multidrug resistance (MDR) in doxorubicin (DOX)-resistant human breast carcinoma cell line MCF/DOX.
Methods
Cells of a human breast cancer cell line, MCF, and its DOX-resistant variant, MCF/DOX, were cultivated with DOX and /or CQ11. The cytotoxicity of drugs in vitro was assayed by MTT method. The accumulation of DOX in these cells was detected by fluorescence spectrophotometer.
Results
MCF/DOX cells were 119 times more resistant to DOX in comparison with MCF cells. After simultaneous treatment with CQ11 at the concentrations of 1.0, 2.5 and 5.0 μmol/L, the IC50 of DOX for MCF/DOX cells decreased from 3.1 ± 0.47 μmol/L to 0.58 ± 0.032, 0.19 ± 0.012 and 0.081 ± 0.015 μmol/L, respectively, thus, increasing the DOX sensitivity by 5.3-fold (P < 0.01), 16-fold (P < 0.01) and 38-fold (P < 0.01), respectively. In the accumulation assay of DOX, simultaneous incubation of MCF/DOX cells with CQ11 significantly increased the DOX accumulation in MCF/DOX cells. No such results were found in parental MCF cells.
Conclusion
CQ11 had strong MDR reversal effect by enhancing intracellular DOX accumulation in MCF/DOX cells, indicating that CQ11 may be a promising MDR chemosensitivity.
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Supported by grants from the National Natural Science Foundation of China (No. 39800181), the Research Foundation of Jiaxing Science and Technology Bureau (No. 2007AY2033) and the Research Foundation of Jiaxing College (No. 70107032).
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Wu, D., Ma, S., Sui, F. et al. Reversal of CQ11, a chloroquine derivative, on multidrug resistance in doxorubicin-resistant breast cancer cell line MCF/DOX. Chin. -Ger. J. Clin. Oncol. 7, 647–649 (2008). https://doi.org/10.1007/s10330-008-0114-z
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DOI: https://doi.org/10.1007/s10330-008-0114-z