Acibenzolar-S-methyl activates stomatal-based defense against Pseudomonas cannabina pv. alisalensis in cabbage
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Pseudomonas cannabina pv. alisalensis (Pcal), which causes bacterial blight of brassicaceous plants, is an economically important pathogen worldwide. Copper fungicide and antibiotics are major strategies to manage the disease caused by Pcal; however, a Pcal strain resistant to these chemicals has already been found, and severe outbreaks of bacterial blight have been reported on cabbage in Japan. Therefore, there is an urgent need to develop new Pcal management strategies. Plant defense activators could be useful not only to protect plants against invading pathogens, but also to reduce the amount of copper fungicides and antibiotics applied. However, the mechanisms by which plant defense activators contribute to controlling diseases remains unclear. In this work, we focused on cabbage and acibenzolar-S-methyl (ASM), a well-known plant defense activator. Expression profiles revealed that ASM induced expression of systemic acquired resistance (SAR) marker genes including PR1, PR2, and PR5 in cabbage plants. We also demonstrated that a soil drench with ASM 2 h before transplanting clearly reduced bacterial blight symptoms and reduced Pcal bacterial populations in cabbage. ASM application was also able to prime cabbage for Pcal resistance by activating stomatal-based defense. Our findings highlight that ASM protects plants from bacterial pathogens by activating stomatal-based defense.
KeywordsAcibenzolar-S-methyl Pseudomonas cannabina pv. alisalensis Cabbage Stomatal-based defense Plant defense activator
We thank Dr. Christina Baker for editing the manuscript. Pcal was kindly provided by the Nagano Vegetable and Ornamental Crops Experiment Station, Nagano, Japan. This work was supported in part by the JST ERATO NOMURA Microbial Community Control Project, JST, Japan.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Human participants or animals
This article does not contain any studies with human participants or animals performed by any of the authors.
- Chitrakar R, Melotto M (2010) Assessing stomatal response to live bacterial cells using whole leaf imaging. J Vis Exp 44:e2185Google Scholar
- Görlach J, Volrath S, Knauf-Beiter G, Hengy G, Beckhove U, Kogel KH, Oostendorp M, Staub T, Ward E, Kessmann H, Ryals J (1996) Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat. Plant Cell 8l:629–643.Google Scholar
- Horinouchi H (2010) Occurrence and control of root browning symptom of Japanese radish caused by Pseudomonas syringae pv. maculicola (in Japanese). Plant Prot (Shokubutsu Boueki) 64:220–223Google Scholar
- Sarris PF, Trantas EA, Baltrus DA, Bull CT, Wechter WP, Yan S, Ververidis F, Almeida NF, Jones CD, Dangl JL, Panopoulos NJ, Vinatzer BA, Goumas DE (2013) Comparative genomics of multiple strains of Pseudomonas cannabina pv. alisalensis, a potential model pathogen of both monocots and dicots. PLoS ONE 8:59366CrossRefGoogle Scholar
- Takahashi F, Ochiai M, Ikeda K, Takikawa Y (2013a) Streptomycin and copper resistance in Pseudomonas cannabina pv. alisalensis (abstract in Japanese). Jpn J Phytopathol 79:35Google Scholar
- Uknes S, Mauch-Mani B, Moyer M, Potter S, Williams S, Dincher S, Chandler D, Slusarenko A, Ward E, Ryals J (1992) Acquired resistance in Arabidopsis. Plant Cell 4:645–656Google Scholar
- Vlot AC, Liu PP, Cameron RK, Park SW, Yang Y, Kumar D, Zhou F, Padukkavidana T, Gustafsson C, Pichersky E, Klessig DF (2008) Identification of likely orthologs of tobacco salicylic acid-binding protein 2 and their role in systemic acquired resistance in Arabidopsis thaliana. Plant J 56:445–456CrossRefGoogle Scholar