Differential gene expression analysis of Secreted in Xylem (SIX) genes from Fusarium oxysporum f. sp. cubense tropical race 4 in Musa acuminata cv. Berangan and potential application for early detection of infection

  • Nee Kiew Poon
  • Chee How Teo
  • Rofina Yasmin OthmanEmail author
Fungal Diseases


Homologues of Secreted in Xylem (SIX) genes, a group of known fungal effectors, were here identified in the genome of Fusarium oxysporum f. sp. cubense tropical race 4 (FocTR4). Expression of FocSIX genes was analyzed in FocTR4 vegetative mycelia and FocTR4-infected banana roots using quantitative reverse transcription PCR (qRT-PCR). Differential gene expression of all FocSIX genes identified in the genome was assessed over time in banana roots inoculated with FocTR4. Two groups of FocSIX genes expression pattern were found; Group I (FocSIX8a, FocSIX1b, FocSIX1c and FocSIX2) represents FocSIX genes that were only expressed in planta, and Group II consists of the remaining FocSIX genes (FocSIX1a, FocSIX6, FocSIX9 and FocSIX13) that were expressed in vitro and in planta. With the FocTR4 genomic information, Group I members can potentially serve as biomarkers for the early detection of Fusarium wilt infection in banana.


Fusarium oxysporum f. sp. cubense Banana Gene expression Secreted in Xylem (SIX) gene Fusarium wilt 



We thank Dr. Santosh Kumar M. Shetty, Miss Kamilatulhusna Zaidi and Mr. Wong Gwo Rong for technical advice. This study was funded by University of Malaya HIR-MOE (Cycle 3) Grant-UM.C/625/1/HIR/MOE/SC/14/4, Postgraduate Research Grant (PPP)-PG178-2016A, BKP078-2016 and RU006-2017. The first author was funded by MyBrainSc scholarships from the Malaysian Ministry of Higher Education.

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Faculty of Science, Institute of Biological SciencesUniversity of MalayaKuala LumpurMalaysia
  2. 2.Centre for Research in Biotechnology for Agriculture (CEBAR)University of MalayaKuala LumpurMalaysia

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