Differential gene expression analysis of Secreted in Xylem (SIX) genes from Fusarium oxysporum f. sp. cubense tropical race 4 in Musa acuminata cv. Berangan and potential application for early detection of infection
- 31 Downloads
Homologues of Secreted in Xylem (SIX) genes, a group of known fungal effectors, were here identified in the genome of Fusarium oxysporum f. sp. cubense tropical race 4 (FocTR4). Expression of FocSIX genes was analyzed in FocTR4 vegetative mycelia and FocTR4-infected banana roots using quantitative reverse transcription PCR (qRT-PCR). Differential gene expression of all FocSIX genes identified in the genome was assessed over time in banana roots inoculated with FocTR4. Two groups of FocSIX genes expression pattern were found; Group I (FocSIX8a, FocSIX1b, FocSIX1c and FocSIX2) represents FocSIX genes that were only expressed in planta, and Group II consists of the remaining FocSIX genes (FocSIX1a, FocSIX6, FocSIX9 and FocSIX13) that were expressed in vitro and in planta. With the FocTR4 genomic information, Group I members can potentially serve as biomarkers for the early detection of Fusarium wilt infection in banana.
KeywordsFusarium oxysporum f. sp. cubense Banana Gene expression Secreted in Xylem (SIX) gene Fusarium wilt
We thank Dr. Santosh Kumar M. Shetty, Miss Kamilatulhusna Zaidi and Mr. Wong Gwo Rong for technical advice. This study was funded by University of Malaya HIR-MOE (Cycle 3) Grant-UM.C/625/1/HIR/MOE/SC/14/4, Postgraduate Research Grant (PPP)-PG178-2016A, BKP078-2016 and RU006-2017. The first author was funded by MyBrainSc scholarships from the Malaysian Ministry of Higher Education.
Compliance with ethical standards
Conflicts of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Czislowski E, Fraser-Smith S, Zander M, O’Neill WT, Meldrum RA, Tran-Nguyen LTT, Batley J, Aitken EAB (2018) Investigation of the diversity of effector genes in the banana pathogen, Fusarium oxysporum f. sp. cubense, reveals evidence of horizontal gene transfer. Mol Plant Pathol 19:1155–1171CrossRefGoogle Scholar
- Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
- Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98Google Scholar
- Li C, Shao J, Wang Y, Li W, Guo D, Yan B, Xia Y, Peng M (2013a) Analysis of banana transcriptome and global gene expression profiles in banana roots in response to infection by race 1 and tropical race 4 of Fusarium oxysporum f. sp. cubense. BMC Genomics 14:851. https://doi.org/10.1186/1471-2164-14-51 CrossRefGoogle Scholar
- Ma LJ, van der Does HC, Borkovich KA, Coleman JJ, Daboussi MJ, Di Pietro A, Dufresne M, Freitag M, Grabherr M, Henrissat B, Houterman PM, Kang S, Shim WB, Woloshuk C, Xie X, Xu JR, Antoniw J, Baker SE, Bluhm BH, Breakspear A, Brown DW, Butchko RA, Chapman S, Coulson R, Coutinho PM, Danchin EG, Diener A, Gale LR, Gardiner DM, Goff S, Hammond-Kosack KE, Hilburn K, Hua-Van A, Jonkers W, Kazan K, Kodira CD, Koehrsen M, Kumar L, Lee YH, Li L, Manners JM, Miranda-Saavedra D, Mukherjee M, Park G, Park J, Park SY, Proctor RH, Regev A, Ruiz-Roldan MC, Sain D, Sakthikumar S, Sykes S, Schwartz DC, Turgeon BG, Wapinski I, Yoder O, Young S, Zeng Q, Zhou S, Galagan J, Cuomo CA, Kistler HC, Rep M (2010) Comparative genomics reveals mobile pathogenicity chromosomes in Fusarium. Nature 464:367–373CrossRefGoogle Scholar
- Mohamed AA, Mak C, Liew KW, Ho YW (1999) Early evaluation of banana plants at nursery stage for fusarium wilt tolerance. In: Molina AB, Nik Masdek NH, Liew KW (eds) Seminar on banana Fusarium wilt management towards sustainable cultivation, Pahang, Malaysia. International Network for the Improvement of Banana and Plantain, Montpellier, pp 174–185Google Scholar
- Rep M, van der Does HC, Meijer M, van Wijk R, Houterman PM, Dekker HL, de Koster CG, Cornelissen BJ (2004) A small, cysteine-rich protein secreted by Fusarium oxysporum during colonization of xylem vessels is required for I-3-mediated resistance in tomato. Mol Microbiol 53:1373–1383CrossRefGoogle Scholar
- Thatcher LF, Williams AH, Garg G, Buck SG, Singh KB (2017) Transcriptome analysis of the fungal pathogen Fusarium oxysporum f. sp. medicaginis during colonisation of resistant and susceptible Medicago truncatula hosts identifies differential pathogenicity profiles and novel candidate effectors. BMC Genomics 17:860CrossRefGoogle Scholar
- Widinugraheni S, Niño-Sánchez J, van der Does HC, van Dam P, García-Bastidas FA, Subandiyah S, Meijer HJG, Kistler HC, Kema GHJ, Rep M (2018) A SIX1 homolog in Fusarium oxysporum f.sp. cubense tropical race 4 contributes to virulence towards Cavendish banana. PLoS One 13:e0205896CrossRefGoogle Scholar
- Yin XM, Xu BY, Zheng W, Wang Z, Wang BZ, Ha WH, Mao HT, Li JY, Sheng ZW, Wang DX, He YD, Han LN, Chen SW, Zhang JB, Fu YG, Wang JB, Jin ZQ (2011) Characterisation of early events in banana roots infected with green fluorescent protein-tagged Fusarium oxysporum f. sp. cubense. Acta Hortic 897:371–376CrossRefGoogle Scholar
- Zheng SJ, García-Bastidas FA, Li X, Zeng L, Bai T, Xu S, Yin K, Li H, Fu G, Yu Y, Yang L, Nguyen HC, Douangboupha B, Khaing AA, Drenth A, Seidl MF, Meijer HJG, Kema GHJ (2018) New geographical insights of the latest expansion of Fusarium oxysporum f.sp. cubense tropical race 4 into the greater Mekong subregion. Front Plant Sci 9:457CrossRefGoogle Scholar