Journal of General Plant Pathology

, Volume 85, Issue 6, pp 413–423 | Cite as

Pseudomonas grimontii, causal agent of turnip bacterial rot disease in Japan

  • Hiroyuki SawadaEmail author
  • Harukuni Horita
  • Tomoo Misawa
  • Yuichi Takikawa
Bacterial and Phytoplasma Diseases


From April to May 2012, a rot disease on turnips grown in greenhouses in southern Hokkaido, Japan caused severe leaf blight, brown discoloration of crown surface, and browning and rot of the interior of main roots. From diseased tissues, bacteria that formed pale beige- to cream-colored colonies were isolated and demonstrated to be pathogenic on turnip after inoculation and reisolation of the isolates. They were gram-negative, aerobic rods with one to three polar flagella, and identified as Pseudomonas grimontii based on biochemical and physiological characterizations, multilocus sequence analysis using concatenated sequences of 16S rRNA, rpoD, gyrB, and rpoB genes, and MALDI-TOF MS analysis using the Bruker MALDI Biotyper system. To the best of our knowledge, P. grimontii has not been isolated previously in Japan, so this study might be the first report of the species in Japan. Although the significance of P. grimontii as a plant pathogenic bacterium has not yet been fully evaluated, we proved in this study that the bacterium is the causal agent of the disease and that its virulence is strong. These findings indicate that it is necessary to record P. grimontii as a novel pathogen posing a threat to plants and requiring vigilance.


Pseudomonas grimontii Brassica rapa var. rapa Bacterial rot MLSA MALDI Biotyper LOPAT 



We deeply appreciate Ms. C. Aoyagi and Ms. H. Nakajima (Genetic Resources Center, NARO) for their daily technical assistance. We also thank Mr. K. Takada (Central Agricultural Experiment Station, Hokkaido Research Organization), Ms. S. Yuuki (Hokkaido Prefectural Sorachi Agricultural Extension Center) and Mr. A. Hagiwara (Hokkaido College of Agriculture) for help with this work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human and animal rights

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10327_2019_869_MOESM1_ESM.docx (42.5 mb)
Supplementary file1 (DOCX 43515 kb)


  1. Anonymous (2012) Common names of plant diseases in Japan, 2nd edn (in Japanese). The Phytopathological Society of Japan, TokyoGoogle Scholar
  2. Anonymous (2018) Common names of plant diseases in Japan, 2018 edn (in Japanese). The Phytopathological Society of Japan, TokyoGoogle Scholar
  3. Anzai Y, Kudo Y, Oyaizu H (1997) The phylogeny of the genera Chryseomonas, Flavimonas, and Pseudomonas supports synonymy of these three genera. Int J Syst Bacteriol 47:249–251CrossRefGoogle Scholar
  4. Baïda N, Yazourh A, Singer E, Izard D (2002) Pseudomonas grimontii sp. nov. Int J Syst Evol Microbiol 52:1497–1503Google Scholar
  5. Bradbury JF (1986) Guide to plant pathogenic bacteria. CAB International, Farnham Royal, SloughGoogle Scholar
  6. Cai TM, Chen LW, Wu SZ, Qian LH, Ren Q (2010) Selection of denitrifying phosphorus-removing bacteria and its characteristic (in Chinese with English summary). Huan Jing Ke Xue 31:2487–2492Google Scholar
  7. Chu XL, Yang B (2010) Isolation and identification of a new pathogen causing soft rot of Phalaenopsis amabilis (in Chinese with English summary). Acta Phytopathol Sin 40:90–94Google Scholar
  8. Cunty A, Poliakoff F, Rivoal C, Cesbron S, Fischer-Le Saux M, Lemaire C, Jacques MA, Manceau C, Vanneste JL (2015) Characterization of Pseudomonas syringae pv. actinidiae (Psa) isolated from France and assignment of Psa biovar 4 to a de novo pathovar: Pseudomonas syringae pv. actinidifoliorum pv. nov. Plant Pathol 64:582–596CrossRefGoogle Scholar
  9. Gomes LC, Piard JC, Briandet R, Mergulhao FJ (2017) Pseudomonas grimontii biofilm protects food contact surfaces from Escherichia coli colonization. Lwt Food Sci Technol 85:309–315CrossRefGoogle Scholar
  10. Horita H, Misawa T, Takada K, Yuuki S, Hagiwara A (2014) Bacterial rot of turnip caused by Pseudomonas marginalis pv. marginalis (in Japanese). Jpn J Phytopathol 80:57Google Scholar
  11. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefGoogle Scholar
  12. Lelliott RA, Billing E, Hayward AC (1966) A determinative scheme for the fluorescent plant pathogenic pseudomonads. J Appl Microbiol 29:470–489Google Scholar
  13. Mulet M, Lalucat J, García-Valdés E (2010) DNA sequence-based analysis of the Pseudomonas species. Environ Microbiol 12:1513–1530Google Scholar
  14. Park YD, Lee HB, Yi H, Kim Y, Bae KS, Choi JE, Jung HS, Chun J (2005) Pseudomonas panacis sp. nov., isolated from the surface of rusty roots of Korean ginseng. Int J Syst Evol Microbiol 55:1721–1724CrossRefGoogle Scholar
  15. Parte AC (2018) LPSN—List of prokaryotic names with standing in nomenclature (, 20 years on. Int J Syst Evol Microbiol 68:1825–1829CrossRefGoogle Scholar
  16. Sawada H, Azegami K (2014) First report of root mat (hairy root) of tomato (Lycopersicon esculentum) caused by Rhizobium radiobacter harboring cucumopine Ri plasmid in Japan (in Japanese with English summary). Jpn J Phytopathol 80:98–114CrossRefGoogle Scholar
  17. Sawada H, Kunugi Y, Watauchi K, Kudo A, Sato T (2011) Bacterial spot, a new disease of grapevine (Vitis vinifera) caused by Xanthomonas  arboricola (in Japanese with English summary). Jpn J Phytopathol 77:7–22CrossRefGoogle Scholar
  18. Sawada H, Shimizu S, Miyoshi T, Shinozaki T, Kusumoto S, Noguchi M, Naridomi T, Kikuhara K, Kansako M, Fujikawa T, Nakaune R (2015) Characterization of biovar 3 strains of Pseudomonas syringae pv. actinidiae isolated in Japan (in Japanese with English summary). Jpn J Phytopathol 81:111–126CrossRefGoogle Scholar
  19. Sawada H, Kondo K, Nakaune R (2016) Novel biovar (biovar 6) of Pseudomonas syringae pv. actinidiae causing bacterial canker of kiwifruit (Actinidia deliciosa) in Japan (in Japanese with English summary). Jpn J Phytopathol 82:101–115CrossRefGoogle Scholar
  20. Sawada H, Fujikawa T, Kita N, Orihara N, Shinozaki T, Shimizu S, Nakaune R, Takikawa Y (2017) Characteristics of Pseudomonas syringae pv. actinidifoliorum causing bacterial leaf spot of Actinidia spp. in Japan (in Japanese with English summary). Jpn J Phytopathol 83:136–150CrossRefGoogle Scholar
  21. Sawada H, Nagai T, Aoki T (2018) Re-identification of strains belonging to the genus Pseudomonas preserved in the Genebank project, NARO (in Japanese). Plant Prot 72:305–314Google Scholar
  22. Schaad NW, Jones JB, Chun W (eds) (2001) Laboratory guide for identification of plant pathogenic bacteria, 3rd edn. APS Press, St. PaulGoogle Scholar
  23. Sudarsono S, Elina J, Giyanto Sukma D (2018) Pathogen causing Phalaenopsis soft rot disease – 16S rDNA and virulence characterisation. Plant Prot Sci 54:1–8Google Scholar
  24. Uad I, Silva-Castro GA, Pozo C, González-López J, Calvo C (2010) Biodegradative potential and characterization of bioemulsifiers of marine bacteria isolated from samples of seawater, sediment and fuel extracted at 4000 m of depth (Prestige wreck). Int Biodeterior Biodegrad 64:511–518CrossRefGoogle Scholar
  25. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, Chun J (2017) Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 67:1613–1617CrossRefGoogle Scholar
  26. Zhou JN, Lin BR, Shen HF, Pu XM, Chen ZN, Feng JJ (2012) First report of a soft rot of Phalaenopsis aphrodita caused by Dickeya dieffenbachiae in China. Plant Dis 96:760CrossRefGoogle Scholar

Copyright information

© The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Genetic Resources CenterNational Agriculture and Food Research Organization (NARO)TsukubaJapan
  2. 2.Central Agricultural Experiment StationHokkaido Research OrganizationNaganumaJapan
  3. 3.Donan Agricultural Experiment StationHokkaido Research OrganizationHokutoJapan
  4. 4.Graduate School of Science and TechnologyShizuoka UniversityShizuokaJapan

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