First report of Tomato leaf curl Joydebpur virus infecting French bean (Phaseolus vulgaris L.)
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During 2016-2017 in Bhagalpur, Bihar, northern India, French bean plants developed curling and yellow mosaic of leaves and stunting. In eight of 12 symptomatic plants, PCR tests for Mungbean yellow mosaic India virus were positive. In the four PCR-negative symptomatic plants, rolling circle amplification (RCA) detected a ~ 2.7-kb DNA that was identified as genomic DNA of Tomato leaf curl Joydebpur virus. Also a ~ 1.4-kb specific PCR product was identified as Tomato leaf curl Joydebpur betasatellite. Whitefly-mediated transmission to chili was as efficient as that to French bean. Our findings provide evidence of a monopartite begomovirus in French bean.
KeywordsBean Begomovirus Betasatellite Leaf curl Whitefly
The present work is supported by Science and Engineering Research Board, Department of Science and Technology, Government of India, Young Scientist Scheme-YSS/2015/000923. The manuscript was developed under BAU communication No. 572/2019.
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Conflict of interest
The authors declare that they have no conflict of interest.
Research involving human participants and/or animals
This article does not contain any studies with human participants or animals performed by any of the authors.
- Agnihotri AK, Mishra SP, Tripathi RC, Ansar M, Srivastava A, Tripathi IP (2018) First natural co-occurrence of tomato leaf curl New Delhi virus DNA-A and chili leaf curl betasatellite on tomato plants (Solanum lycopersicum L.) in India. J Gen Plant Pathol 84:414–417. https://doi.org/10.1007/s10327-018-0807-2 CrossRefGoogle Scholar
- Bernardo P, Golden M, Akram M, Naimuddin Nadarajan N, Fernandez E, Granier M, Rebelo AG, Peterschmitt M, Martin DP, Roumagnac P (2013) Identification and characterisation of a highly divergent geminivirus: evolutionary and taxonomic implications. Virus Res 177:35–45. https://doi.org/10.1016/j.virusres.2013.07.006 CrossRefPubMedGoogle Scholar
- Brown JK, Zerbini FM, Navas-Castillo J, Moriones E, Ramos-Sobrinho R, Silva JCF, Fiallo-Olive E, Briddon RW, Hernandez-Zepeda C, Idris A, Malathi VG, Martin DP, Rivera-Bustamante R, Ueda S, Varsani A (2015) Revision of Begomovirus taxonomy based on pairwise sequence comparisons. Arch Virol 160:1593–1619CrossRefGoogle Scholar
- Deng A, McGrath PF, Robinson DJ, Harrison BD (1994) Detection and differentiation of whitefly transmitted geminiviruses in plants and vector insects by the polymerase chain reaction with degenerate primers. Ann Appl Biol 125:327–336. https://doi.org/10.1111/j.1744-7348.1994.tb04973.x CrossRefGoogle Scholar
- Malathi VG, John P (2008) Characterization, diagnosis and management of plant viruses: vegetables and pulses crops. In: Rao GP, Lava Kumar P, Holguin-Pena RJ (eds) Plant pathogens series. Studium Press, Houston, pp 97–123Google Scholar
- Muniyappa V, Reddy HR, Shivshankar C (1975) Yellow mosaic disease of Dolichos biflorus Linn. Curr Res 4:176Google Scholar
- Muniyappa V, Venkatesh HM, Ramappa HK, Kulkarni RS, Zeidan M, Tarba CY, Ghanim M, Czosnek H (2000) Tomato leaf curl virus from Bangalore (ToLCV-Ban4): sequence comparison with Indian ToLCV isolates, detection in plants and insects, and vector relationships. Arch Virol 145:1583–1598. https://doi.org/10.1007/s007050070078 CrossRefPubMedGoogle Scholar
- Naimuddin K, Akram M, Pratap A, Chaubey BR, John JK (2011) PCR based identification of the virus causing yellow mosaic disease in wild Vigna accessions. J Food Legu 24:14–17Google Scholar
- Naimuddin K, Akram M, Singh NP (2016) Yellow mosaic of mungbean and urdbean: current status and future strategies. J Food Legu 29:77–93Google Scholar
- Varma A, Malathi VG (2003) Emerging geminivirus problems: a serious threat to crop production. Ann Appl Biol 142:145–164. https://doi.org/10.1111/j.1744-7348.2003.tb00240.x CrossRefGoogle Scholar
- Venkataravanappa V, Swarnalatha P, Lakshminarayana Reddy CN, Mahesh B, Rai AB, Krishna Reddy M (2012) Molecular evidence for association of Tobacco curly shoot virus and a betasatellite with curly shoot disease of common bean (Phaseolus vulgaris L.) from India. J Plant Pathol Microbiol 3:148–156. https://doi.org/10.4172/2157-7471.1000148 CrossRefGoogle Scholar