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A class III peroxidase PRX34 is a component of disease resistance in Arabidopsis

  • Lei Zhao
  • Le Thi Phuong
  • Mai Thanh Luan
  • Aprilia Nur Fitrianti
  • Hidenori Matsui
  • Hirofumi Nakagami
  • Yoshiteru Noutoshi
  • Mikihiro Yamamoto
  • Yuki Ichinose
  • Tomonori Shiraishi
  • Kazuhiro ToyodaEmail author
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Abstract

PRX34 mediates the oxidative burst in Arabidopsis. Here we characterized two additional Arabidopsis prx34 null mutants (prx34-2, prx34-3), besides the well-studied prx34-1. Due to a decrease in corresponding peroxidase, the activity that generates reactive oxygen species (ROS) was significantly lower in cell wall extracts of prx34-2 and prx34-3 plants. Consistently, the prx34-2 and prx34-3 exhibited reduced accumulation both of ROS and callose in Flg22-elicitor-treated leaves, leading to enhanced susceptibility to bacterial and fungal pathogens. In contrast, ectopic expression of PRX34 in the wild type caused enhanced resistance. PRX34 is thus a component for disease resistance in Arabidopsis.

Keywords

Apoplastic oxidative burst Arabidopsis Cell wall Class III peroxidase PRX34 Reactive oxygen species (ROS) 

Notes

Acknowledgements

We acknowledge Prof. Dr. Shinji Tsuyumu (Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University, Shizuoka, Japan) for generously providing Pectobacterium carotovorum subsp. carotovorum strain Pc1. This research was supported in part by the Grants-in-Aid for Scientific Research (18K05645) from the Japan Society for Promotion of Science (JSPS).

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflict of interest.

Research involving human and/or animal participants

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

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Supplementary material 1 (DOCX 22 kb)
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Copyright information

© The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  • Lei Zhao
    • 1
  • Le Thi Phuong
    • 1
  • Mai Thanh Luan
    • 1
  • Aprilia Nur Fitrianti
    • 1
  • Hidenori Matsui
    • 1
    • 2
  • Hirofumi Nakagami
    • 2
    • 3
  • Yoshiteru Noutoshi
    • 1
  • Mikihiro Yamamoto
    • 1
  • Yuki Ichinose
    • 1
  • Tomonori Shiraishi
    • 1
    • 4
  • Kazuhiro Toyoda
    • 1
    Email author
  1. 1.Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Environmental and Life ScienceOkayama UniversityOkayamaJapan
  2. 2.RIKEN Center for Sustainable Resource ScienceYokohamaJapan
  3. 3.Laboratory of Basic Immune System of Plants/Protein Mass SpectrometryMax Planck Institute for Plant Breeding ResearchCologneGermany
  4. 4.Research Institute of Biological Sciences (RIBS)OkayamaJapan

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