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Genetic diversity of Phytophthora palmivora isolates from Indonesia and Japan using rep-PCR and microsatellite markers

  • Masanto
  • Ayaka Hieno
  • Arif WibowoEmail author
  • Siti Subandiyah
  • Masafumi Shimizu
  • Haruhisa Suga
  • Koji Kageyama
Fungal Diseases
  • 35 Downloads

Abstract

Phytophthora palmivora infects hundreds of plant species around the world. Determining the genetic diversity among isolates is necessary to establish more effective control strategies, manage resistance and understand epidemiology, long-term evolution of the pathogen, host–pathogen coevolution, and evolutionary processes. Here genetic diversity among 81 isolates of P. palmivora from various host plants and geographical regions in Indonesia and Japan was evaluated using rep-PCR (BOX, ERIC, REP and M13) and microsatellite markers. With both methods, the isolates clustered into six groups, which corresponded more to geographic regions rather than host plants or mating types, although the respective members of each group were not always consistent between the two methods. Isolates from Indonesia, Japan and other countries clearly clustered into separate groups in the rep-PCR analysis. The rep-PCR consistently grouped the isolates from Sumatera Island with some isolates from Sulawesi, but microsatellite markers grouped them with those from the Java islands. A typical A1-mating type from one region grouped in one cluster using both methods. Both DNA fingerprinting markers also grouped the isolates from Nusa Tenggara and Papua islands with those from Japan. Genetic variation was recognized within and among populations of P. palmivora based on geographical areas, host plants and mating types. Possible explanations for the results and suggested strategies for disease management are discussed.

Keywords

Phytophthora palmivora Genetic diversity Rep-PCR Microsatellite 

Notes

Acknowledgements

This research was part of doctoral study of Masanto with the collaboration of the Faculty of Agriculture, University of Gadjah Mada, Yogyakarta (Indonesia) and River Basin Research Center, Gifu University, Gifu (Japan) as part of the “6-months Sandwich Program in UGSAS-GU”. The assistance of Mrs. Kayoko Otsubo is gratefully acknowledged.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human/animal rights statement

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  • Masanto
    • 1
    • 5
  • Ayaka Hieno
    • 2
  • Arif Wibowo
    • 1
    Email author
  • Siti Subandiyah
    • 1
    • 6
  • Masafumi Shimizu
    • 3
  • Haruhisa Suga
    • 4
  • Koji Kageyama
    • 2
  1. 1.Faculty of AgricultureUniversity of Gadjah MadaYogyakartaIndonesia
  2. 2.River Basin Research CenterGifu UniversityGifuJapan
  3. 3.Faculty of Applied Biological ScienceGifu UniversityGifuJapan
  4. 4.Life Science Research CenterGifu UniversityGifuJapan
  5. 5.Tanjung Priok Agricultural Quarantine Agency, Ministry of AgricultureJakartaIndonesia
  6. 6.Research Center for BiotechnologyUniversitas Gadjah MadaYogyakartaIndonesia

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