The p27 open reading frame of tomato infectious chlorosis virus encodes a suppressor of RNA silencing
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Tomato infectious chlorosis virus (TICV) belongs to the genus Crinivirus in the family Closteroviridae. We used Agrobacterium co-infiltration leaf patch assays to investigate the suppression of RNA silencing by p27, which is encoded by TICV-RNA1 ORF2 in a genomic location similar to that of viral suppressor of RNA silencing (VSR) ORF in other criniviruses. Additionally, Nicotiana benthamiana was inoculated with a potato virus X vector (pGR106) carrying the p27 sequence (pGR106–p27) to examine whether p27 is a VSR. Plants infiltrated with pGR106–p27 exhibited more severe necrosis and mosaic symptoms. This is the first report describing a TICV VSR.
KeywordsClosteroviridae Crinivirus Tomato infectious chlorosis virus RNA silencing suppressor
This study was supported in part by JSPS KAKENHI [Grant-in-Aid for Scientific Research (B), Grant numbers 26304023 and 17H04617]. We thank Edanz Group (http://www.edanzediting.com/ac) for editing a draft of this manuscript.
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The authors declare that they have no conflict of interest.
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This article does not contain any studies with human participants or animals performed by any of the authors.
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