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Journal of General Plant Pathology

, Volume 85, Issue 4, pp 301–305 | Cite as

The p27 open reading frame of tomato infectious chlorosis virus encodes a suppressor of RNA silencing

  • Takaaki Mashiko
  • Wei-Qin Wang
  • Sedyo Hartono
  • Gede Suastica
  • Yutaro Neriya
  • Hisashi Nishigawa
  • Tomohide NatsuakiEmail author
Viral and Viroid Diseases
  • 533 Downloads

Abstract

Tomato infectious chlorosis virus (TICV) belongs to the genus Crinivirus in the family Closteroviridae. We used Agrobacterium co-infiltration leaf patch assays to investigate the suppression of RNA silencing by p27, which is encoded by TICV-RNA1 ORF2 in a genomic location similar to that of viral suppressor of RNA silencing (VSR) ORF in other criniviruses. Additionally, Nicotiana benthamiana was inoculated with a potato virus X vector (pGR106) carrying the p27 sequence (pGR106–p27) to examine whether p27 is a VSR. Plants infiltrated with pGR106–p27 exhibited more severe necrosis and mosaic symptoms. This is the first report describing a TICV VSR.

Keywords

Closteroviridae Crinivirus Tomato infectious chlorosis virus RNA silencing suppressor 

Notes

Acknowledgements

This study was supported in part by JSPS KAKENHI [Grant-in-Aid for Scientific Research (B), Grant numbers 26304023 and 17H04617]. We thank Edanz Group (http://www.edanzediting.com/ac) for editing a draft of this manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human rights and animal participants

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Biological Production Science, United Graduate School of Agricultural ScienceTokyo University of Agriculture and TechnologyFuchuJapan
  2. 2.School of AgricultureUtsunomiya UniversityUtsunomiyaJapan
  3. 3.Gadjahmada UniversityYogyakartaIndonesia
  4. 4.Bogor Agricultural UniversityBogorIndonesia

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