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Journal of General Plant Pathology

, Volume 85, Issue 4, pp 251–256 | Cite as

Pathogenicity of Phytophthora palmivora to fig fruits after its long-term storage in water

  • Noriyuki MiyakeEmail author
  • Hirofumi Nagai
Technique
  • 577 Downloads

Abstract

Long-term storage methods for Phytophthora palmivora were tested using five isolates stored in sterile distilled water (SDW) for up to 6 years and 8 months, and their growth and virulence were evaluated. All five cultures remained viable in SDW at room temperature and retained normal mycelial growth characteristics and morphology. All but one culture formed normal zoospores, and all five were still pathogenic to fig fruits. These findings suggest that this SDW storage is a simple, inexpensive method for long-term preservation of the pathogenic oomycete in the laboratory.

Keyword

Ficus carica L. Long-term storage Pathogenicity Phytophthora palmivora Viability Water 

Notes

Acknowledgements

We thank Dr. Rie Gappa-Adachi for providing the PP1-1 and PP3 isolates.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10327_2019_847_MOESM1_ESM.docx (20 kb)
Supplementary material 1 (DOCX 19 KB)
10327_2019_847_MOESM2_ESM.docx (986 kb)
Supplementary material 2 (DOCX 986 KB)

References

  1. Boesewinkel HJ (1976) Storage of fungal cultures in water. Trans Br Mycol Soc 66:183–185CrossRefGoogle Scholar
  2. Borman AM, Szekely A, Campbell CK, Johnson EM (2006) Evaluation of the viability of pathogenic filamentous fungi after prolonged storage in sterile water and review of recent published studies on storage methods. Mycopathologia 161:361–368CrossRefPubMedGoogle Scholar
  3. Castellani A (1963) Further researches on the long viability and growth of many pathogenic fungi and some bacteria in sterile distilled water. Mycopathol Mycol Appl 20:1–6CrossRefPubMedGoogle Scholar
  4. Dahmen H, Staub TH, Schwinn FJ (1983) Technique for long-term preservation of phytopathogenic fungi in liquid nitrogen. Phytopathology 73:241–246 CrossRefGoogle Scholar
  5. El-Gholl NE, Alfieri SA Jr (1984) Fruit rot of fig caused by Phytophthora palmivora. Proc Fla State Hort Soc 97:327–328Google Scholar
  6. Erwin DC, Ribeiro OK (eds) (1996) Phytophthora diseases worldwide. APS Press, St. PaulGoogle Scholar
  7. Gappa-Adachi R, Yano K, Takeuchi S, Morita Y, Uematsu S (2012) Phytophthora blight of southern star (Oxypetalum caeruleum) caused by Phytophthora palmivora in Japan. J Gen Plant Pathol 78:39–42CrossRefGoogle Scholar
  8. Hori S (1915) Phytophthora fici (in Japanese). J Plant Prot 2:930–932Google Scholar
  9. Ko WH (2003) Long-term storage and survival structure of three species of Phytophthora in water. J Gen Plant Pathol 69:186–188Google Scholar
  10. Ko WH, Chase LL, Kunimoto RK (1973) A microsyringe method for determining concentration of fungal propagules. Phytopathology 63:1206–1207CrossRefGoogle Scholar
  11. Marx DH, Daniel WJ (1976) Maintaining cultures of ectomycorrhizal and plant pathogenic fungi in sterile water cold storage. Can J Microbiol 22:338–341CrossRefPubMedGoogle Scholar
  12. McGinnis MR, Padhye AA, Ajello L (1974) Storage of stock cultures of filamentous fungi, yeasts, and some aerobic Actinomycetes in sterile distilled water. Appl Microbiol 28:218–222PubMedPubMedCentralGoogle Scholar
  13. Miyake N, Ito S (2005) Seasonal occurrence of fruit rots in fig fields (in Japanese). Annu Rep Kansai Plant Protect Soc 47:87–89CrossRefGoogle Scholar
  14. Miyake N, Nagai H (2017) Efficacy of phosphonate in controlling white powdery rot of fig caused by Phytophthora palmivora. J Gen Plant Pathol 83:390–397CrossRefGoogle Scholar
  15. Morita Y, Tojo M (2007) Modifications of PARP medium using fluazinam, miconazole, and nystatin for detection of Pythium spp. in soil. Plant Dis 91:1591–1599CrossRefGoogle Scholar
  16. Richter DL, Bruhn J (1989) Revival of saprotrophic and mycorrhizal basidiomycete cultures from cold storage in sterile water. Can J Microbiol 35:1055–1060CrossRefGoogle Scholar
  17. Simpfendorfe S, Harden TJ, Murray GM (1996) Viability and pathogenicity of Phytophthora clandestina after storage in water and liquid nitrogen. Australas Plant Pathol 25:234–239CrossRefGoogle Scholar
  18. Smith D (1982) Liquid nitrogen storage of fungi. Trans Br Mycol Soc 79:415–421CrossRefGoogle Scholar
  19. Zhang C, Zhang W, Ma HQ, Zhang GZ (2013) First report of Phytophthora palmivora causing fruit rot of fig (Ficus carica L.) in China. Plant Dis 97:1252CrossRefPubMedGoogle Scholar

Copyright information

© The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Aichi Agricultural Research CenterNagakuteJapan

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