Pathogenicity of Phytophthora palmivora to fig fruits after its long-term storage in water
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Long-term storage methods for Phytophthora palmivora were tested using five isolates stored in sterile distilled water (SDW) for up to 6 years and 8 months, and their growth and virulence were evaluated. All five cultures remained viable in SDW at room temperature and retained normal mycelial growth characteristics and morphology. All but one culture formed normal zoospores, and all five were still pathogenic to fig fruits. These findings suggest that this SDW storage is a simple, inexpensive method for long-term preservation of the pathogenic oomycete in the laboratory.
KeywordFicus carica L. Long-term storage Pathogenicity Phytophthora palmivora Viability Water
We thank Dr. Rie Gappa-Adachi for providing the PP1-1 and PP3 isolates.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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