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Journal of General Plant Pathology

, Volume 85, Issue 3, pp 201–210 | Cite as

Phytophthora sojae zoospores differ in chemotaxis to the root and root exudates of host soybean and nonhost common bean

  • Zhuoqun Zhang
  • Ying Xu
  • Guangmei Song
  • Xinying Gao
  • Yuqi Zhao
  • Mengzhen Jia
  • Yufei Chen
  • Bing Suo
  • Qiuming Chen
  • Di Wu
  • Wenxu Wu
  • Jingzhi WenEmail author
Fungal Diseases
  • 216 Downloads

Abstract

Phytophthora root and stem rot of soybean is a destructive disease in many countries caused by the soil-borne pathogen Phytophthora sojae. The interaction between soil-borne pathogens and plant roots before invasion is a focus of interest for revealing host and nonhost resistance mechanisms to soil-borne pathogens. In the present study, soybean cvs. Sloan (susceptible), Williams 82 (resistant) and nonhost common bean cv. Yidianhong were used to determine the effect of roots, root exudates, and isoflavones, amino acids, sugars, and citric acid from the root exudates on the pre-infection behavior (taxis, encystment and cyst germination) of zoospores of P. sojae. The elongation zone of roots of both host soybeans attracted significantly more zoospores than did that of the nonhost bean, and that of the susceptible cultivar attracted significantly more zoospores than did that of the resistant cultivar. Similarly, the host soybean root exudates attracted zoospores and promoted zoospore encystment and cyst germination, but nonhost root exudates had no effect on zoospores. Thus, P. sojae apparently selects its host depending on the root exudates. Of the 26 total components detected from root exudates, the exudates from susceptible and resistant soybeans and nonhost common bean had 12, 17, and 25 components respectively. The differences between the host and nonhost were mainly in the type and concentration of amino acids. Most components were significant chemoattractants of P. sojae zoospores. However, when these attractants were mixed at their relative concentrations in the root exudates, the chemoattraction was significantly reduced compared to that of the sum of each components, and the reduction from strongest to weakest was nonhost common bean, resistant and susceptible soybeans, indicating that these components in the root exudates interact with each other, and the intensity of the interaction relates to the type and concentration of the components. This phenomenon is because some components may share the same receptor, thus causing an obstructive effect. This result may also be a reason that common bean is a nonhost of P. sojae. In addition, the relative local concentrations of citric acid in the root exudates of both nonhost cv. Yidianhong and resistant cv. Williams 82 were repellent to zoospores of P. sojae. Root exudates of susceptible cv. Sloan amended with two concentrations of citric acids especially reduced zoospore chemoattraction, indicating that citric acid in the root exudates also plays an important role in host and nonhost resistance to P. sojae.

Keywords

Phytophthora sojae Chemotaxis Root exudates Host soybean Nonhost common bean 

Notes

Acknowledgements

This research was supported by the National Natural Science Foundation of China (grant nos. 31670444, 31370449).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human participants or animals

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10327_2019_839_MOESM1_ESM.caj (548 kb)
Supplementary material 1 (CAJ 548 KB)
10327_2019_839_MOESM2_ESM.pdf (829 kb)
Supplementary material 2 (PDF 828 KB)

References

  1. Badri DV, Vivanco JM (2009) Regulation and function of root exudates. Plant Cell Environ 32:666–681CrossRefPubMedGoogle Scholar
  2. Costamilan LM, Clebsch CC, Soares RM, Seixas CDS, Godoy CV, Dorrance AE (2013) Pathogenic diversity of Phytophthora sojae pathotypes from Brazil. Eur J Plant Pathol 135:845–853CrossRefGoogle Scholar
  3. Donaldson SP, Deacon JW (1993) Effects of amino acids and sugars on zoospore taxis, encystment and cyst germination in Pythium aphanidermatum (Edson) Fitzp., P. catenulatum Matthews and P. dissotocum Drechs. New Phytol 123:289–295CrossRefGoogle Scholar
  4. Dong Y, Dong K, Zheng Y, Yang Z, Tang L, Xiao J (2014) Allelopathic effects and components analysis of root exudates of faba bean cultivars with different degrees of resistance to Fusarium oxysporum (in Chinese with English summary). Chin. J Eco-Agr 22:292–299Google Scholar
  5. Dorrance AE, Mills D, Robertson AE, Draper MA, Giesler L, Tenuta A (2007) Phytophthora root and stem rot of soybean. The Plant Health Instructor.  https://doi.org/10.1094/PHI-I-2007-0830-07 (Reviewed 2012)CrossRefGoogle Scholar
  6. Graham TL (1991) Flavonoid and isoflavonoid distribution in developing soybean seedling tissues and in seed and root exudates. Plant Physiol 95:594–603CrossRefPubMedPubMedCentralGoogle Scholar
  7. Hao WY, Ran W, Shen QR, Ren LX (2010) Effects of root exudates from watermelon, rice plants and phenolic acids on Fusarium oxysporum f. sp. niveum (in Chinese with English summary). Sci Agric Sin 43:2443–2452Google Scholar
  8. Hua C, Yang X, Wang Y (2015) Phytophthora sojae and soybean isoflavones, a model to study zoospore chemotaxis. Physiol Mol Plant Pathol 92:161–165CrossRefGoogle Scholar
  9. Huang WB, Ma R, Yang D, Liu XP, Song JF (2014) Organic acids secreted from plant roots under soil stress and their effects on ecological adaptability of plants. Agric Sci Technol 15:1167–1173Google Scholar
  10. Morris PF, Ward EWB (1992) Chemoattraction of zoospores of the soybean pathogen, Phytophthora sojae, by isoflavones. Physiol Mol Plant Pathol 40:17–22CrossRefGoogle Scholar
  11. Riggs K (2010) Chemotaxis of Phytophthora sojae zoospores to soybean roots is altered by isoflavone silencing. Honors thesis, Ohio State University, Columbus, OH, USAGoogle Scholar
  12. Schmitthenner AF, Bhat RG (1994) Useful methods for studying Phytophthora in the laboratory. Special circular 143. Ohio Agricultural Research Development Center, WoosterGoogle Scholar
  13. Subramanian S, Graham MY, Yu O, Graham TL (2005) RNA interference of soybean isoflavone synthase genes leads to silencing in tissues distal to the transformation site and to enhanced susceptibility to Phytophthora sojae. Plant Physiol 137:1345–1353CrossRefPubMedPubMedCentralGoogle Scholar
  14. Suo B, Chen Q, Wu W, Wu D, Tian M, Jie Y, Zhang B, Wen J (2016) Chemotactic responses of Phytophthora sojae zoospores to amino acids and sugars in root exudates. J Gen Plant Pathol 82:142–148CrossRefGoogle Scholar
  15. Tyler BM (2002) Molecular basis of recognition between Phytophthora pathogens and their hosts. Annu Rev Phytopathol 40:137–167CrossRefPubMedGoogle Scholar
  16. Tyler BM (2007) Phytophthora sojae: root rot pathogen of soybean and model oomycete. Mol Plant Pathol 8:1–8CrossRefGoogle Scholar
  17. Uren NC (2000) Types, amounts, and possible functions of compounds released into the rhizosphere by soil-grown plants. In: Pinton R, Varanini Z, Nannipieri P (eds) The rhizosphere: biochemistry and organic substances at the soil–plant interface. Marcel Dekker, New York, pp 19–40Google Scholar
  18. Wang JY, Lv YY, Yu D, Zhang WN, Pu FZ, Shen SS (2014) Effects of root exudates from different resistant pepper varieties on Phytophthora capsici (in Chinese with English summary). China Vegetables 1:13–16Google Scholar
  19. Wang YL, Almvik M, Clarke N, Eich-Greatorex S, Øgaard AF, Krogstad T, Lambers H, Clarke JL (2015) Contrasting responses of root morphology and root-exuded organic acids to low phosphorus availability in three important food crops with divergent root traits. AoB Plants 7:1–11CrossRefGoogle Scholar
  20. Xu BH, Xie LX, Zhang LY (2014) Advanced in group irritability phenomenon in fungi (in Chinese with English summary). J Microbiol 34:100–105Google Scholar
  21. Zhang XB, Wu Y, Lin H (2006) Study on method of hydrolyze isoflavone in soybean by HPLC (in Chinese with English summary). Cereals Oils 4:19–21Google Scholar
  22. Zhang L, Fang Y, Ji S, Jiao Y, Liao J, Li J, Deng W, Zhu S, Yin J, Yang M (2015) Inhibitory activity of maize root exudates against Phytophthora nicotianae and antifungal compounds analysis (in Chinese with English summary). Chinese J Biol Control 31:115–122Google Scholar

Copyright information

© The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  • Zhuoqun Zhang
    • 1
  • Ying Xu
    • 1
  • Guangmei Song
    • 1
  • Xinying Gao
    • 1
  • Yuqi Zhao
    • 1
  • Mengzhen Jia
    • 1
  • Yufei Chen
    • 1
  • Bing Suo
    • 1
  • Qiuming Chen
    • 1
  • Di Wu
    • 1
  • Wenxu Wu
    • 1
  • Jingzhi Wen
    • 1
    Email author
  1. 1.Department of Plant Protection, College of AgricultureNortheast Agricultural UniversityHarbinPeople’s Republic of China

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