Detection of Pythium porphyrae infecting Philippine Pyropia acanthophora based on morphology and nuclear rRNA internal transcribed spacer sequences
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Outbreaks of Pythium porphyrae, a fungus that causes red rot disease can devastate nori production, but studies have focused on the occurrence of P. porphyrae from cultivated hosts compared with the wild ones. An unexpected encounter of P. porphyrae infecting the wild nori seaweed, Pyropia acanthophora, collected from the Philippines prompted the present study. The definitive morphological diagnoses of P. porphyrae infection based from the previous reports were similar to those obtained here. Phylogenetic and haplotype analyses of nuclear rRNA internal transcribed spacer sequences confirmed that the pathogen infecting the Philippine Py. acanthophora can be assigned to P. porphyrae.
KeywordsFungal pathogen Nori Nuclear rRNA ITS Pythium porphyrae Pyropia acanthophora
I thank Z. Aguinaldo for field and laboratory assistance. R. Andres, C. Abiva, and C. Ame of the BFAR-RO2 organized the collection trip in Claveria, Cagayan. The comments from two anonymous reviewers are greatly acknowledged. Additional comments and edits from B. E. Hazen greatly improved the quality of the manuscript. Support was provided by the Far Eastern University, University Research Center (FEU-URC) and by a grant from the Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development (DOST-PCAARRD) Science Education Institute through an Accelerated Science and Technology Human Resources Development Program (DOST-SEI ASTHRDP).
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Conflict of interest
The author declares that he has no conflict of interest.
This article does not contain any studies with human participants or animals performed by the author.
- Ame EC, Ayson JP, Okuda K, Andres R (2010) Porphyra fisheries in the northern Philippines: some environmental issues and the socio-economic impact on the Ilocano fisherfolk. Kuroshio Sci 4:53–58Google Scholar
- Bolboli Z, Mostowfizadeh-Ghalamfarsa R (2015) Phylogenetic relationships and taxonomic characteristics of Pythium spp. isolates in cereal fields of Fars Province. Iran J Plant Pathol 51:471–492Google Scholar
- De Cock AWAM (1986) Marine Pythiaceae from decaying seaweeds in the Netherlands. Mycotaxon 25:101–110Google Scholar
- Kammarnjesadakul P, Palaga T, Sritunyalucksana K, Mendoza L, Krajaenjun T, Vanittanakom N, Tongchusak S, Denduangboripant J, Chindamporn A (2011) Phylogenetic analysis of Pythium insidiosum Thai strains using cytochrome oxidase II (COX II) DNA coding sequences and internal transcribed spacer regions (ITS). Med Mycol 49:289–295CrossRefGoogle Scholar
- Lodhi AM, Shahzad S, Ghaffar A (2004) Re-description of Pythium adhaerens Sparrow. Pak J Bot 36:453–456Google Scholar
- Miller MA, Pfeiffer W, Schwartz T (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the gateway computing environments workshops (GCE). New Orleans, pp 1–8Google Scholar
- Robideau GP, De Cock AWAM, Coffey MD, Voglmayr H, Brouwer H, Bala K, Chitty DW, Désaulniers N, Eggertson QA, Gachon CMM, Hu CH, Küpper FC, Rintoul TL, Sarhan E, Verstappen ECP, Zhang Y, Bonants PJM, Ristaino JB, Lévesque CA (2011) DNA barcoding of oomycetes with cytochrome c oxidase subunit I and internal transcribed spacer. Mol Ecol Resour 11:1002–1011CrossRefGoogle Scholar
- Rojas JA, Jacobs JL, Napieralski S, Karaj B, Bradley CA, Chase T, Esker PD, Giesler LJ, Jardine DJ, Malvick DK, Markell SG, Nelson BD, Robertson AE, Rupe JC, Smith DL, Sweets LE, Tenuta AU, Wise KA, Chilvers MI (2017) Oomycete species associated with soybean seedlings in North America—part I: identification and pathogenicity characterization. Phytopathology 107:280–292CrossRefGoogle Scholar
- Salmaninezhad F, Mostowfizadeh-Ghalamfarsa R (2017) Phylogeny of Phytophthora and Phytopythium species associated with rice in Fars Province (Iran). Rostaniha 18:1–15Google Scholar
- Takahashi M, Ichitani T, Sasaki M (1977) Pythium porphyrae Takahashi et Sasaki, sp. nov. causing red rot of marine red algae Porphyra spp. Trans Mycol Soc Jpn 18:279–285Google Scholar