Evaluation of various cultivars of Actinidia species and breeding source Actinidia rufa for resistance to Pseudomonas syringae pv. actinidiae biovar 3
- 109 Downloads
Pseudomonas syringae pv. actinidiae causes kiwifruit bacterial canker, which seriously harms or kills plants, in many countries. After the first appearance of P. syringae pv. actinidiae biovar 3, having the strongest virulence among all biovars of this pathogen, in Japanese orchards in 2014, the disease spread widely and damaged plants across the nation within a few years. Since introduction of resistant cultivars is the best approach to control the disease, we examined the responses of nine kiwifruit cultivars and six collection lines used as genetic resources for breeding after infiltration of detached leaves with P. syringae pv. actinidiae biovar 3. All cultivars had different degrees of resistance to the pathogen, and collection lines of Actinidia rufa and cultivar Kosui crossed with A. rufa and A. chinensis showed significant resistance. Our investigation revealed that A. rufa is an excellent genetic source for kiwifruit breeding.
KeywordsKiwifruit Bacterial canker Psa3 Disease resistance Pseudomonas syringae pv. actinidiae Actinidia rufa
We acknowledge Shinichi Shimizu, Fruit Tree Research Center, Ehime Research Institute of Agriculture, Forestry and Fisheries, Ehime Prefecture for valuable advice on developing evaluation methods and identifying P. syringae pv. actinidiae biovar 3 and Daniel G. Panaccione for English editing. This research was supported by grants from the Project of the NARO Bio-oriented Technology Research Advancement Institution (the special scheme project on regional developing strategy) (No. 16822474).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Human and animal rights statement
This article does not contain any studies with human participants or animals performed by any of the authors.
- Balestra GM, Mazzaglia A, Quattrucci A, Renzi M, Rossetti A (2009) Current status of bacterial canker spread on kiwifruit in Italy. Australas Plant Dis Notes 4:34–36Google Scholar
- Cunty A, Poliakoff F, Rivoal C, Cesbron S, Fischer-Le Saux M, Lemaire C, Jacques MA, Manceau C, Vanneste JL (2015) Characterization of Pseudomonas syringae pv. actinidiae (Psa) isolated from France and assignment of Psa biovar 4 to a de novo pathovar: Pseudomonas syringae pv. actinidifoliorum pv. nov. Plant Pathol 64:582–596CrossRefGoogle Scholar
- Fukuda T (2009) The situation and cultivar tend of kiwifruits [in Japanese]. J Home Econ Jpn 60:913–917Google Scholar
- McCann HC, Rikkerink EHA, Bertels F, Fiers M, Lu A, Rees-George J, Andersen MT, Gleave AP, Haubold B, Wohlers MW, Guttman DS, Wang PW, Straub C, Vanneste JL, Rainey PB, Templeton MD (2013) Genomic analysis of the kiwifruit pathogen Pseudomonas syringae pv. actinidiae provides insight into the origins of an emergent plant disease. PLoS Pathog 9:e1003503CrossRefGoogle Scholar
- Phivnil K, Beppu K, Mochioka R, Fukuda T, Kataoka I (2004) Low-chill trait for endodormancy completion in Actinidia arguta Planch. (Sarunashi) and A. rufa Planch. (Shima-sarunashi), indigenous Actinidia species in Japan and their interspecific hybrids. J Japan Soc Hort Sci 73:244–246CrossRefGoogle Scholar