Chitin and chitosan-based support materials for enzyme immobilization and biotechnological applications

  • Madan L. VermaEmail author
  • Sandeep Kumar
  • Anamika Das
  • Jatinder S. Randhawa
  • Munusamy Chamundeeswari


Enzymes of industrial importance are primarily employed for biotechnological applications. However, high-cost and instability issues of purified enzymes hamper their usage. Multiple reuses rather than the single use is more cost-effective. A robuster bioprocess is feasible by enzyme immobilization. Performance of immobilized enzymes depends on the nature of support materials. Chitin and its derivatives-based supports offer stability and cost-effective bioprocessing. Chitosan is biocompatible, biodegradable, non-toxic and has multiple functional groups. A variety of supports such as chitosan, chitosan film, chitosan nanoparticle and chitosan nanocomposite are employed for enzyme immobilization. Chitosan bound enzymes, as compared to free enzymes, have improved the biocatalytic performances due to exceptionally high operational stability and reusability. Here we review enzymes immobilized on chitin/chitosan supporting materials with applications ranging from agriculture to drug delivery.


Chitosan Enzymes Immobilization Reusability Drug delivery Sustainable agriculture 



Authors would like to thank the Director, Indian Institute of Information Technology Una, Himachal Pradesh for providing the necessary facility to carry out the present work.


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Authors and Affiliations

  1. 1.Department of Biotechnology, School of Basic SciencesIndian Institute of Information TechnologyUnaIndia
  2. 2.Teva API (TAPI)Greater NoidaIndia
  3. 3.University College of Paramedical SciencesGuru Kashi UniversityTalwandi SaboIndia
  4. 4.Centre for Environmental Sciences and TechnologyCentral University of PunjabBathindaIndia
  5. 5.Department of BiotechnologySt. Joseph’s College of EngineeringSholinganallur, ChennaiIndia

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