Environmental Chemistry Letters

, Volume 18, Issue 1, pp 229–235 | Cite as

A non-derivatized method for simultaneous quantitation of proteinogenic, urea-cycle, and acetylated amino acids by liquid chromatography–high-resolution mass spectrometry

  • Annaleise R. Klein
  • Krista A. Barzen-Hanson
  • Ludmilla AristildeEmail author
Original Paper


The quantification of amino acids as freely dissolved compounds or from hydrolyzed peptides and proteins is a common endeavor in biomedical and environmental sciences. In order to avoid the drawbacks of derivatization and application challenges of tandem mass spectrometry, we present here a robust 13-min liquid chromatography coupled with a full-scan mass spectrometry method to achieve rapid detection and quantification of 30 amino acids without derivatization. We combined hydrophilic interaction liquid chromatography with heated electrospray ionization and high-resolution mass spectrometry operated with polarity switching to analyze the 20 proteinogenic amino acids, ornithine, citrulline, homoserine, cystine, and six acetylated amino acids. We obtained high mass accuracy and good precision of the targeted amino acids. Limits of detection ranged from 0.017 to 1.3 µM. Noteworthy for environmental samples, we found comparable ionization efficiency and quantitative detection for the majority of the analytes prepared with pure water versus a high-salt solution. We applied the method to profile carbon source-dependent secretions of amino acids by Pseudomonas protegens Pf-5, a well-known plant-beneficial bacterium.


Polarity switching Non-targeted analysis Acetylated amino acids Urea-cycle amino acids High-resolution mass spectrometry 



Postdoctoral supports for A.R.K. and K.B-H. were provided, respectively, by a National Science Foundation CAREER Grant (award # 1653092) and a research fellowship from Cornell University, both awarded to L. A. We thank Rebecca A. Wilkes (Aristilde Research Group, Cornell University) for preparing the bacterial cultures.

Compliance with ethical standards

Conflicts of Interest

The authors declare no conflicts of interest.

Supplementary material

10311_2019_927_MOESM1_ESM.pdf (1.2 mb)
Supplementary file1 (PDF 1193 kb)


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of Biological and Environmental EngineeringCornell UniversityIthacaUSA
  2. 2.Department of Civil and Environmental EngineeringNorthwestern UniversityEvanstonUSA
  3. 3.Division of Mathematics and Natural SciencesElmira CollegeElmiraUSA

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