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Environmental Chemistry Letters

, Volume 17, Issue 4, pp 1857–1866 | Cite as

Isolation and characterization of hydrophilic dissolved organic matter in waters by ion exchange solid phase extraction followed by high resolution mass spectrometry

  • Wei Wang
  • Chen He
  • Yuan Gao
  • Yahe Zhang
  • Quan ShiEmail author
Original Paper
  • 226 Downloads

Abstract

Dissolved organic matter (DOM) is a complex substance occurring in marine and freshwater environments. DOM has many functions that modify physical, chemical and biological processes in ecological systems. Solid-phase extraction (SPE) is widely used for the separation of DOM. However, a part of strongly hydrophilic compounds are lost using classical SPE separation. In this article, the strong hydrophilic components, which cannot be extracted by the hydrophobic reverse phase SPE, were further extracted in a natural organic matter sample by ion exchange SPE cartridges to obtain hydrophilic acid (HIA), hydrophilic neutral (HIN), and hydrophilic base (HIB) fractions. The extracts were characterized by three-dimensional excitation–emission matrix fluorescence spectroscopy and negative-ion electrospray Fourier transform ion cyclotron resonance mass spectrometry (ESI FT-ICR MS). The results revealed that the hydrophilic components were significantly different from the hydrophobic components in molecular composition. Hydrophilic components were identified as tryptophan-like and tyrosine-like compounds by three-dimensional fluorescence spectra. The HIA fraction contains mainly tannic acid-like compounds with high O/C ratio; the HIB fraction contains mainly amide-like compounds; and the HIN fraction contains mainly lignin and lipids.

Keywords

Dissolved organic matter Hydrophilic Solid-phase extraction Three-dimensional excitation–emission matrix Fourier transform ion cyclotron resonance mass spectrometry 

Abbreviations

DOM

Dissolved organic matter

SPE

Solid-phase extraction

3DEEM

Three-dimensional excitation–emission matrix

ESI FT-ICR MS

Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

HOS

Hydrophobic substance

HIA

Hydrophilic acid

HIN

Hydrophilic neutral

HIB

Hydrophilic base

DBE

Double-bond equivalent

TOC

Total organic matter

1H-NMR

Proton nuclear magnetic resonance

KMD

Kendrick mass defect

Notes

Acknowledgements

This work was supported by the National Key Research and Development Program of China (2018YFA0605800).

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Heavy Oil ProcessingChina University of PetroleumBeijingPeople’s Republic of China

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