An increase in cell membrane permeability in the in situ extractive fermentation improves the production of antroquinonol from Antrodia camphorata S-29


The goals of this study were to increase the production of antroquinonol (AQ) and to elucidate the response mechanism of the cell membrane during the in situ extractive fermentation (ISEF) of Antrodia camphorata S-29. Through ISEF, the concentration of AQ reached a maximum of 146.1 ± 2.8 mg/L, which was approximately (7.4 ± 0.1)-fold that of the control (coenzyme Q0-induced fermentation). Transcriptome sequencing showed that four genes (FAD2, fabG, SCD, and FAS1) related to fatty acid biosynthesis were upregulated. FAD2 and SCD may regulate the increase in oleic acid (C18:1) and linoleic acid (C18:2) in the cell membrane of A. camphorata S-29, resulting in an increase in cell membrane permeability. AQ was successfully transferred to the n-tetradecane phase through the cell membrane, reducing product feedback inhibition and improving the production of AQ from A. camphorata S-29.

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This work was supported by the National Natural Science Foundation of China (Grant No. 31871757), Shanghai Engineering Research Center of Food Microbiology (Grant No. 19 DZ2281100), and the Hujiang Foundation of China (Grant No. D15012).

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Correspondence to Lian-Zhong Ai.

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Liu, X., Xia, Y., Lai, P.F. et al. An increase in cell membrane permeability in the in situ extractive fermentation improves the production of antroquinonol from Antrodia camphorata S-29. J Ind Microbiol Biotechnol (2020).

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  • Antrodia camphorata
  • Antroquinonol
  • In situ extractive fermentation
  • Cell membrane permeability
  • Fatty acid metabolism