Deletion of PHO13 improves aerobic l-arabinose fermentation in engineered Saccharomyces cerevisiae

  • Suji Ye
  • Deokyeol Jeong
  • Jong Cheol Shon
  • Kwang-Hyeon Liu
  • Kyoung Heon Kim
  • Minhye ShinEmail author
  • Soo Rin KimEmail author
Bioenergy/Biofuels/Biochemicals - Short Communication


Pentose sugars are increasingly being used in industrial applications of Saccharomyces cerevisiae. Although l-arabinose is a highlighted pentose that has been identified as next-generation biomass, arabinose fermentation has not yet undergone extensive development for industrial utilization. In this study, we integrated a heterologous fungal arabinose pathway with a deletion of PHO13 phosphatase gene. PHO13 deletion increased arabinose consumption rate and specific ethanol productivity under aerobic conditions and consequently depleted sedoheptulose by activation of the TAL1 gene. Global metabolite profiling indicated upregulation of the pentose phosphate pathway and downstream effects such as trehalose accumulation and downregulation of the TCA cycle. Our results suggest that engineering of PHO13 has ample potential for arabinose conversion to ethanol as an industrial source for biofuels.


l-arabinose PHO13 TAL1 Sedoheptulose 



d-xylose reductase


Xylitol dehydrogenase


d-xylose isomerase



S. cerevisiae

Saccharomyces cerevisiae


Optical density at 600 nm


Real-Time quantitative Polymerase Chain Reaction


Not detected


Ethanol yield


Specific ethanol productivity


Dried cell weight



This work was carried out with support from the “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ01279801)” Rural Development Administration, Republic of Korea.

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflict of interest.

Supplementary material

10295_2019_2233_MOESM1_ESM.docx (2.6 mb)
Supplementary material 1 (DOCX 2713 kb)


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Copyright information

© Society for Industrial Microbiology and Biotechnology 2019

Authors and Affiliations

  1. 1.School of Food Science and BiotechnologyKyungpook National UniversityDaeguRepublic of Korea
  2. 2.Department of Environmental Toxicology Research CenterKorea Institute of ToxicologyJinjuRepublic of Korea
  3. 3.College of Pharmacy and Research Institute of Pharmaceutical SciencesKyungpook National UniversityDaeguRepublic of Korea
  4. 4.Department of Biotechnology, Graduate SchoolKorea UniversitySeoulRepublic of Korea

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