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Promoting microbial utilization of phenolic substrates from bio-oil

  • Kirsten Davis
  • Marjorie R. Rover
  • Davinia Salvachúa
  • Ryan G. Smith
  • Gregg T. Beckham
  • Zhiyou Wen
  • Robert C. Brown
  • Laura R. JarboeEmail author
Bioenergy/Biofuels/Biochemicals - Original Paper

Abstract

The economic viability of the biorefinery concept is limited by the valorization of lignin. One possible method of lignin valorization is biological upgrading with aromatic-catabolic microbes. In conjunction, lignin monomers can be produced by fast pyrolysis and fractionation. However, biological upgrading of these lignin monomers is limited by low water solubility. Here, we address the problem of low water solubility with an emulsifier blend containing approximately 70 wt% Tween® 20 and 30 wt% Span® 80. Pseudomonas putida KT2440 grew to an optical density (OD600) of 1.0 ± 0.2 when supplied with 1.6 wt% emulsified phenolic monomer-rich product produced by fast pyrolysis of red oak using an emulsifier dose of 0.076 ± 0.002 g emulsifier blend per g of phenolic monomer-rich product. This approach partially mitigated the toxicity of the model phenolic monomer p-coumarate to the microbe, but not benzoate or vanillin. This study provides a proof of concept that processing of biomass-derived phenolics to increase aqueous availability can enhance microbial utilization.

Keywords

Pseudomonas putida KT2440 Lignin Bio-oil Emulsion Phenols 

Notes

Acknowledgements

Funding for this research was provided by Iowa State University’s Bioeconomy Institute, and NSF Energy for Sustainability, award number CBET-1605034. This work was also authored in part by Alliance for Sustainable Energy, LLC, the manager and operator of the National Renewable Energy Laboratory for the U.S. Department of Energy (DOE) under Contract no. DE-AC36-08GO28308. Funding to DS and GTB was provided by the U.S. Department of Energy Office of Energy Efficiency and Renewable Energy Technologies Office.

Compliance with ethical standards

Conflict of interest

There are no conflicts of interest.

Supplementary material

10295_2019_2208_MOESM1_ESM.pdf (237 kb)
Supplementary material 1 (PDF 236 kb)

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Copyright information

© Society for Industrial Microbiology and Biotechnology 2019

Authors and Affiliations

  1. 1.Chemical and Biological Engineering, 4134 Biorenewable Research LaboratoryIowa State UniversityAmesUSA
  2. 2.Bioeconomy InstituteIowa State UniversityAmesUSA
  3. 3.National Bioenergy CenterNational Renewable Energy LaboratoryGoldenUSA
  4. 4.Department of Food Science and Human NutritionIowa State UniversityAmesUSA

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