Journal of Industrial Microbiology & Biotechnology

, Volume 46, Issue 11, pp 1517–1529 | Cite as

Mild hydrothermal pretreatment of sugarcane bagasse enhances the production of holocellulases by Aspergillus niger

  • Caio de Oliveira Gorgulho SilvaEmail author
  • Agenor de Castro Moreira dos Santos Júnior
  • Renata Henrique Santana
  • Ricardo Henrique Krüger
  • Wagner Fontes
  • Marcelo Valle de Sousa
  • Carlos André Ornelas Ricart
  • Edivaldo Ximenes Ferreira Filho
Bioenergy/Biofuels/Biochemicals - Original Paper


Holocellulase production by Aspergillus niger using raw sugarcane bagasse (rSCB) as the enzyme-inducing substrate is hampered by the intrinsic recalcitrance of this material. Here we report that mild hydrothermal pretreatment of rSCB increases holocellulase secretion by A. niger. Quantitative proteomic analysis revealed that pretreated solids (PS) induced a pronounced up-regulation of endoglucanases and cellobiohydrolases compared to rSCB, which resulted in a 10.1-fold increase in glucose release during SCB saccharification. The combined use of PS and pretreatment liquor (PL), referred to as whole pretreated slurry (WPS), as carbon source induced a more balanced up-regulation of cellulases, hemicellulases and pectinases and resulted in the highest increase (4.8-fold) in the release of total reducing sugars from SCB. The use of PL as the sole carbon source induced the modulation of A. niger’s secretome towards hemicellulose degradation. Mild pretreatment allowed the use of PL in downstream biological operations without the need for undesirable detoxification steps.


Integrated enzyme production Biorefinery Proteomics Secretome Holocellulose-degrading enzymes 



This work was supported by the Brazilian National Council for Scientific and Technological Development (CNPq), the Coordination for the Improvement of Higher Education Personnel (CAPES) and the Foundation for Research Support of the Federal District (FAPDF, Grant PRONEX 0193.001195/2016). Funding was also provided by the Funding Authority for Studies and Projects (FINEP/CT-INFRA, Grants 0439/2011 and 0694/2013) to MVS.

Supplementary material

10295_2019_2207_MOESM1_ESM.pdf (799 kb)
Supplementary material 1 (PDF 798 kb)
10295_2019_2207_MOESM2_ESM.pdf (724 kb)
Supplementary material 2 (PDF 723 kb)


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Copyright information

© Society for Industrial Microbiology and Biotechnology 2019

Authors and Affiliations

  • Caio de Oliveira Gorgulho Silva
    • 1
    Email author
  • Agenor de Castro Moreira dos Santos Júnior
    • 2
  • Renata Henrique Santana
    • 3
  • Ricardo Henrique Krüger
    • 1
  • Wagner Fontes
    • 2
  • Marcelo Valle de Sousa
    • 2
  • Carlos André Ornelas Ricart
    • 2
  • Edivaldo Ximenes Ferreira Filho
    • 1
  1. 1.Laboratory of Enzymology, Department of Cellular BiologyUniversity of BrasíliaBrasíliaBrazil
  2. 2.Laboratory of Protein Chemistry and Biochemistry, Department of Cellular BiologyUniversity of BrasíliaBrasíliaBrazil
  3. 3.Instituto Federal de BrasíliaBrasíliaBrazil

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