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The complete genomic sequence of Streptomyces spectabilis NRRL-2792 and identification of secondary metabolite biosynthetic gene clusters

  • Arkadeep Sinha
  • Silvia Phillips-Salemka
  • Tanu-Adhikari Niraula
  • Kevin A. Short
  • Narayan P. NiraulaEmail author
Natural Products - Original Paper

Abstract

This is the first report of a fully annotated genomic sequence of Streptomyces spectabilis NRRL-2792, isolated and identified by The Upjohn Company in 1961. The genome was assembled into a single scaffold for annotation and analysis. The chromosome is linear, 9.5 Mb in size which is one of the largest Streptomyces genomes yet described, has a G+C content of 72%, and encodes for approximately 7943 genes. Antibiotic Secondary Metabolite Analysis Shell (antiSMASH) and Basic Local Alignment Search Tool (BLAST) bioinformatics analyses identified six complete secondary metabolite biosynthetic gene clusters for ectoine, melanin, albaflavenone, spectinomycin, 2-methylisoborneol and coelichelin. Additionally, biosynthetic clusters were identified that shared ≥ 90% gene content with complestatin, hopene, neoaureothin, or undecylprodigiosin. Thirty-one other likely secondary metabolite gene clusters were identified by antiSMASH. BLAST identified two subsets of undecylprodigiosin biosynthetic genes at polar opposites of the chromosome; their duplication was subsequently confirmed by primer walking.

Keywords

Antibiotics DNA sequencing Secondary metabolites Streptomyces 

Notes

Acknowledgements

We appreciate the review and suggestions of Michael J. White and Richard H. Baltz.

Supplementary material

10295_2019_2172_MOESM1_ESM.docx (532 kb)
Supplementary material 1 (DOCX 531 kb)

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Copyright information

© Society for Industrial Microbiology and Biotechnology 2019

Authors and Affiliations

  1. 1.Bioprocess Development GroupPfizerKalamazooUSA

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