Metabolic engineering of glucose uptake systems in Corynebacterium glutamicum for improving the efficiency of l-lysine production

  • Jian-Zhong XuEmail author
  • Hai-Bo Yu
  • Mei Han
  • Li-Ming Liu
  • Wei-Guo Zhang
Metabolic Engineering and Synthetic Biology - Original Paper


Traditional amino acid producers typically exhibit the low glucose uptake rate and growth deficiency, resulting in a long fermentation time because of the accumulation of side mutations in breeding of strains. In this study, we demonstrate that the efficiency of l-lysine production in traditional l-lysine producer Corynebacterium glutamicum ZL-9 can be improved by rationally engineering glucose uptake systems. To do this, different bypasses for glucose uptake were investigated to reveal the best glucose uptake system for l-lysine production in traditional l-lysine producer. This study showed that overexpression of the key genes in PTSGlc or non-PTSGlc increased the glucose consumption, growth rate, and l-lysine production. However, increasing the function of PTSGlc in glucose uptake led to the increase of by-products, especially for plasmid-mediated expression system. Increasing the participation of non-PTSGlc in glucose utilization showed the best glucose uptake system for l-lysine production. The final strain ZL-92 with increasing the expression level of iolT1, iolT2 and ppgK could produce 201.6 ± 13.8 g/L of l-lysine with a productivity of 5.04 g/L/h and carbon yield of 0.65 g/(g glucose) in fed-batch culture. This is the first report of a rational modification of glucose uptake systems that improve the efficiency of l-lysine production through increasing the participation of non-PTSGlc in glucose utilization in traditional l-lysine producer. Similar strategies can be also used for producing other amino acids or their derivatives.


Corynebacterium glutamicum Glucose uptake system Glucose consumption rate l-Lysine production efficiency 



This work was supported by the National Natural Science Foundation of China [Grant Number 31601459], the China Postdoctoral Science Foundation [Grant Number 2016M590410], and the National First-class Discipline Program of Light Industry Technology and Engineering [Grant Number LITE2018-07]. We thank Dr. XL Chen (State Key Laboratory of Food Science and Technology at Jiangnan University) for assistance in data analysis.

Supplementary material

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Supplementary material 1 (DOCX 1141 kb)


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Copyright information

© Society for Industrial Microbiology and Biotechnology 2019

Authors and Affiliations

  1. 1.The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of BiotechnologyJiangnan UniversityWuxiPeople’s Republic of China
  2. 2.Shanghai Business SchoolShanghaiPeople’s Republic of China
  3. 3.State Key Laboratory of Food Science and Technology, School of BiotechnologyJiangnan UniversityWuxiPeople’s Republic of China

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