High-yield production of l-serine from glycerol by engineered Escherichia coli
- 82 Downloads
l-Serine is widely used in pharmaceutical, food and cosmetic industries, and the direct fermentation to produce l-serine from cheap carbon sources such as glycerol is greatly desired. The production of l-serine by engineered Escherichia coli from glycerol has not been achieved so far. In this study, E. coli was engineered to efficiently produce l-serine from glycerol. To this end, three l-serine deaminase genes were deleted in turn, and all of the deletions caused the maximal accumulation of l-serine at 0.06 g/L. Furthermore, removal of feedback inhibition by l-serine resulted in a titer of 1.1 g/L. Additionally, adaptive laboratory evolution was employed to improve glycerol utilization in combination with the overexpression of the cysteine/acetyl serine transporter gene eamA, leading to 2.36 g/L l-serine (23.6% of the theoretical yield). In 5-L bioreactor, l-serine titer could reach up to 7.53 g/L from glycerol, demonstrating the potential of the established strain and bioprocess.
KeywordsAdaptive laboratory evolution Escherichia coli Genetic engineering Glycerol l-Serine
This work was financially supported by the National Natural Science Foundation of China (Nos. 31300027, 31600044), the Natural Science Foundation of Jiangsu Province (No. BK20150151).
- 1.Al-Rabiee R, Lee EJ, Grant GA (1996) The mechanism of velocity modulated allosteric regulation in D-3-phosphoglycerate dehydrogenase cross-linking adjacent regulatory domains with engineered disulfides mimics effector binding. J Biol Chem 271:13013–13017. https://doi.org/10.1074/jbc.271.22.13013 CrossRefPubMedGoogle Scholar
- 12.Mundhada H, Seoane JM, Schneider K, Koza A, Christensen HB, Klein T, Phaneuf PV, Herrgard M, Feist AM, Nielsen AT (2017) Increased production of l-serine in Escherichia coli through adaptive laboratory evolution. Metab Eng 39:141–150. https://doi.org/10.1016/j.ymben.2016.11.008 CrossRefPubMedGoogle Scholar
- 16.Wang Y, Li Q, Zheng P, Guo Y, Wang L, Zhang T, Sun J, Ma Y (2016) Evolving the l-lysine high-producing strain of Escherichia coli using a newly developed high-throughput screening method. J Ind Microbiol Biotechnol 43:1227–1235. https://doi.org/10.1007/s10295-016-1803-1 CrossRefPubMedPubMedCentralGoogle Scholar
- 17.Wendisch VF, Lindner SN, Meiswinkel TM (2011) Use of glycerol in biotechnological applications. In: Montero G (ed) Biodiesel-quality, emissions and by-products. InTech. https://doi.org/10.5772/25338
- 20.Xu G, Zhu Q, Luo Y, Zhang X, Guo W, Dou W, Li H, Xu H, Zhang X, Xu Z (2015) Enhanced production of l-serine by deleting sdaA combined with modifying and overexpressing serA in a mutant of Corynebacterium glutamicum SYPS-062 from sucrose. Biochem Eng J 103:60–67. https://doi.org/10.1016/j.bej.2015.06.009 CrossRefGoogle Scholar