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Purification and characterization of indochrome type blue pigment produced by Pseudarthrobacter sp. 34LCH1 isolated from Atacama desert

  • Sebastian Finger
  • Félix A. GodoyEmail author
  • Geraldine Wittwer
  • Carlos P. Aranda
  • Raúl Calderón
  • Claudio D. Miranda
Natural Products - Original Paper
  • 168 Downloads

Abstract

The interest in and demand for natural dyes has increased significantly in recent years; however, very few natural blue dyes are commercially available, because blue colored compounds in nature are relatively rare. In this study, a blue pigment-producing bacteria from Lake Chungará (Atacama Desert, Chile) was isolated, and its blue pigment was purified and chemically characterized. The pigment-producing strain was identified as Pseudarthrobacter sp. by 16S rRNA gene sequencing. The pigment was separated from the filtered culture medium by column chromatography/solid-phase extraction using different resins (ionic exchange, C-18, size exclusion). The strain produced up to 2.5 g L−1 of blue pigment, which was very soluble in water, partially soluble in methanol and insoluble in other organic solvents. The pigment was analyzed and characterized by analytical HPLC, UV–Vis, FT-IR, and H-NMR, and purified by semi-preparative HPLC. The pigment was non-toxic to brine shrimp (LD50 > 2.3 g L−1) and was stable at pH 6–10 at temperatures below 60 °C. HPLC analysis shows that the pigment is composed of four major blue fractions. The physicochemical properties and structural analysis demonstrate that this pigment belongs to the indochrome isomers, whose properties have yet to have been characterized. The high solubility in water, good stability in neutral and basic pH, and negligible toxicity of the blue pigment make it a good candidate suitable for several industrial and possibly some food applications.

Keywords

Physic-chemical properties Chemical characterization Natural colorant Indochrome Blue dyes Bacterial pigment 

Notes

Acknowledgements

The authors thank Dr. Matthew Lee for his support for the review of this manuscript. This work was supported by Innova CORFO-Chile (IDL2-18532) and Internal Research Project on Applied Science of the Universidad de Los Lagos, not existing conflicts of interest with funding source.

Supplementary material

10295_2018_2088_MOESM1_ESM.docx (282 kb)
Supplementary material 1 (DOCX 281 kb)

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Copyright information

© Society for Industrial Microbiology and Biotechnology 2018

Authors and Affiliations

  • Sebastian Finger
    • 1
  • Félix A. Godoy
    • 2
    Email author
  • Geraldine Wittwer
    • 2
  • Carlos P. Aranda
    • 3
  • Raúl Calderón
    • 4
    • 5
  • Claudio D. Miranda
    • 6
  1. 1.Facultad de Medicina y CienciaUniversidad San SebastiánPuerto MonttChile
  2. 2.Centro i-marUniversidad de Los LagosPuerto MonttChile
  3. 3.Departamento de Ciencias Biológicas y BiodiversidadUniversidad de Los LagosOsornoChile
  4. 4.Centro de Investigación en Recursos Naturales y SustentabilidadUniversidad Bernardo O‘HigginsSantiagoChile
  5. 5.Instituto de Investigaciones Agropecuarias, INIA La PlatinaSantiagoChile
  6. 6.Laboratorio de Patobiología Acuática, Departamento de AcuiculturaUniversidad Católica del NorteCoquimboChile

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