Journal of Industrial Microbiology & Biotechnology

, Volume 45, Issue 12, pp 1045–1051 | Cite as

Enhanced microbial lipid production by Cryptococcus albidus in the high-cell-density continuous cultivation with membrane cell recycling and two-stage nutrient limitation

  • Rongzhan Fu
  • Qiang FeiEmail author
  • Longan Shang
  • Christopher J. Brigham
  • Ho Nam Chang
Fermentation, Cell Culture and Bioengineering - Original Paper


As a potential feedstock for biofuel production, a high-cell-density continuous culture for the lipid production by Cryptococcus albidus was investigated in this study. The influences of dilution rates in the single-stage continuous cultures were explored first. To reach a high-cell-density culture, a single-stage continuous culture coupled with a membrane cell recycling system was carried out at a constant dilution rate of 0.36/h with varied bleeding ratios. The maximum lipid productivity of 0.69 g/L/h was achieved with the highest bleeding ratio of 0.4. To reach a better lipid yield and content, a two-stage continuous cultivation was performed by adjusting the C/N ratio in two different stages. Finally, a lipid yield of 0.32 g/g and lipid content of 56.4% were obtained. This two-stage continuous cultivation, which provided a higher lipid production performance, shows a great potential for an industrial-scale biotechnological production of microbial lipids and biofuel production.


High-cell-density culture Lipid production Membrane cell recycling Two-stage continuous culture Biofuel 



This work was supported by the Key Research and Development Program of Shaanxi Province (2017GY-146), the China postdoctoral science foundation (2017M623206), and the National Research Foundation of Korea Grant (NRF-2011-0009582).


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Copyright information

© Society for Industrial Microbiology and Biotechnology 2018

Authors and Affiliations

  1. 1.School of Chemical EngineeringNorthwest UniversityXi’anChina
  2. 2.School of Chemical Engineering and TechnologyXi’an Jiaotong UniversityXi’anChina
  3. 3.College of Biological and Chemical Engineering, Ningbo Institute of TechnologyZhejiang UniversityNingboChina
  4. 4.Department of BioengineeringUniversity of Massachusetts DartmouthNorth DartmouthUSA
  5. 5.Department of Chemical and Biomolecular EngineeringKAISTDaejeonKorea

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