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Enhanced microbial lipid production by Cryptococcus albidus in the high-cell-density continuous cultivation with membrane cell recycling and two-stage nutrient limitation

  • Rongzhan Fu
  • Qiang Fei
  • Longan Shang
  • Christopher J. Brigham
  • Ho Nam Chang
Fermentation, Cell Culture and Bioengineering - Original Paper
  • 54 Downloads

Abstract

As a potential feedstock for biofuel production, a high-cell-density continuous culture for the lipid production by Cryptococcus albidus was investigated in this study. The influences of dilution rates in the single-stage continuous cultures were explored first. To reach a high-cell-density culture, a single-stage continuous culture coupled with a membrane cell recycling system was carried out at a constant dilution rate of 0.36/h with varied bleeding ratios. The maximum lipid productivity of 0.69 g/L/h was achieved with the highest bleeding ratio of 0.4. To reach a better lipid yield and content, a two-stage continuous cultivation was performed by adjusting the C/N ratio in two different stages. Finally, a lipid yield of 0.32 g/g and lipid content of 56.4% were obtained. This two-stage continuous cultivation, which provided a higher lipid production performance, shows a great potential for an industrial-scale biotechnological production of microbial lipids and biofuel production.

Keywords

High-cell-density culture Lipid production Membrane cell recycling Two-stage continuous culture Biofuel 

Notes

Acknowledgments

This work was supported by the Key Research and Development Program of Shaanxi Province (2017GY-146), the China postdoctoral science foundation (2017M623206), and the National Research Foundation of Korea Grant (NRF-2011-0009582).

References

  1. 1.
    Aggelis G, Komaitis M (1999) Enhancement of single cell oil production by Yarrowia lipolytica growing in the presence of Teucrium polium L. aqueous extract. Biotechnol Lett 21:747–749CrossRefGoogle Scholar
  2. 2.
    Brown BD, Hsu KH, Hammond EG, Glatz BA (1989) A relationship between growth and lipid accumulation in Candida curvata D. J Ferment Bioeng 68:344–352CrossRefGoogle Scholar
  3. 3.
    Chang HN, Jung K, Lee JC, Woo H-C (2014) Multi-stage continuous high cell density culture systems: a review. Biotechnol Adv 32:514–525CrossRefPubMedCentralGoogle Scholar
  4. 4.
    Chang HN, Kim N-J, Kang J, Jeong CM, Fei Q, Kim BJ, Kwon S, Lee SY, Kim J (2011) Multi-stage high cell continuous fermentation for high productivity and titer. Bioprocess Biosyst Eng 34:419–431CrossRefPubMedCentralGoogle Scholar
  5. 5.
    Chang HN, Yoo I-K, Kim BS (1994) High density cell culture by membrane-based cell recycle. Biotechnol Adv 12:467–487CrossRefPubMedCentralGoogle Scholar
  6. 6.
    Cheirsilp B, Kitcha S, Torpee S (2012) Co-culture of an oleaginous yeast Rhodotorula glutinis and a microalga Chlorella vulgaris for biomass and lipid production using pure and crude glycerol as a sole carbon source. Ann Microbiol 62:987–993CrossRefGoogle Scholar
  7. 7.
    Evans CT, Ratledge C (1983) A comparison of the oleaginous yeast, candida curvata, grown on different carbon sources in continuous and batch culture. Lipids 18:623–629CrossRefPubMedCentralGoogle Scholar
  8. 8.
    Fei Q, Chang HN, Shang L (2011) Exploring low-cost carbon sources for microbial lipids production by fed-batch cultivation of Cryptococcus albidus. Biotechnol Bioprocess Eng 16:482–487CrossRefGoogle Scholar
  9. 9.
    Fei Q, Chang HN, Shang L, Kim N, Kang J (2011) The effect of volatile fatty acids as a sole carbon source on lipid accumulation by Cryptococcus albidus for biodiesel production. Bioresour Technol 102:2695–2701CrossRefGoogle Scholar
  10. 10.
    Galafassi S, Cucchetti D, Pizza F, Franzosi G, Bianchi D, Compagno C (2012) Lipid production for second generation biodiesel by the oleaginous yeast Rhodotorula graminis. Bioresour Technol 111:398–403CrossRefPubMedCentralGoogle Scholar
  11. 11.
    Haas C, El-Najjar T, Virgolini N, Smerilli M, Neureiter M (2017) High cell-density production of poly (3-hydroxybutyrate) in a membrane bioreactor. New Biotechnol 37:117–122CrossRefGoogle Scholar
  12. 12.
    Hassan M, Blanc PJ, Granger L-M, Pareilleux A, Goma G (1993) Lipid production by an unsaturated fatty acid auxotroph of the oleaginous yeast Apiotrichum curvatum grown in single-stage continuous culture. Appl Microbiol Biotechnol 40:483–488CrossRefGoogle Scholar
  13. 13.
    Huang W-D, Zhang Y-HP (2011) Analysis of biofuels production from sugar based on three criteria: thermodynamics, bioenergetics, and product separation. Energy Environ Sci 4:784–792CrossRefGoogle Scholar
  14. 14.
    Ienczak JL, Schmidell W, de Aragão GMF (2013) High-cell-density culture strategies for polyhydroxyalkanoate production: a review. J Ind Microbiol Biotechnol 40:275–286.  https://doi.org/10.1007/s10295-013-1236-z CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Jansen ML, van Gulik WM (2014) Towards large scale fermentative production of succinic acid. Curr Opin Biotechnol 30:190–197CrossRefPubMedCentralGoogle Scholar
  16. 16.
    Lee CW, Chang HN (1987) Kinetics of ethanol fermentations in membrane cell recycle fermentors. Biotechnol Bioeng 29:1105–1112CrossRefPubMedCentralGoogle Scholar
  17. 17.
    Lee K, Lefebvre M, Tribe D, Rogers P (1980) High productivity ethanol fermentations with Zymomonas mobilis using continuous cell recycle. Biotechnol Lett 2:487–492CrossRefGoogle Scholar
  18. 18.
    Lee YL, Chang HN (1990) High cell density culture of a recombinant Escherichia coli producing penicillin acylase in a membrane cell recycle fermentor. Biotechnol Bioeng 36:330–337CrossRefPubMedCentralGoogle Scholar
  19. 19.
    Li H, Kim N-J, Jiang M, Kang JW, Chang HN (2009) Simultaneous saccharification and fermentation of lignocellulosic residues pretreated with phosphoric acid–acetone for bioethanol production. Bioresour Technol 100:3245–3251CrossRefPubMedCentralGoogle Scholar
  20. 20.
    Liang Y, Cui Y, Trushenski J, Blackburn JW (2010) Converting crude glycerol derived from yellow grease to lipids through yeast fermentation. Bioresour Technol 101:7581–7586CrossRefPubMedCentralGoogle Scholar
  21. 21.
    Mahendran M, Pedersen S (2004) Method of potting hollow fiber membranes. US6685832B2Google Scholar
  22. 22.
    Meesters PAEP, Huijberts GNM, Eggink G (1996) High-cell-density cultivation of the lipid accumulating yeast Cryptococcus curvatus using glycerol as a carbon source. Appl Microbiol Biotechnol 45:575–579.  https://doi.org/10.1007/s002530050731 CrossRefGoogle Scholar
  23. 23.
    O’Brien DJ, Roth LH, McAloon AJ (2000) Ethanol production by continuous fermentation–pervaporation: a preliminary economic analysis. J Membr Sci 166:105–111CrossRefGoogle Scholar
  24. 24.
    Papanikolaou S, Aggelis G (2002) Lipid production by Yarrowia lipolytica growing on industrial glycerol in a single-stage continuous culture. Bioresour Technol 82:43–49CrossRefPubMedCentralGoogle Scholar
  25. 25.
    Park GW, Fei Q, Jung K, Chang HN, Kim Y-C, N-j Kim, J-d-r Choi, Kim S, Cho J (2014) Volatile fatty acids derived from waste organics provide an economical carbon source for microbial lipids/biodiesel production. Biotechnol J 9:1536–1546CrossRefGoogle Scholar
  26. 26.
    Ratledge C (2002) Regulation of lipid accumulation in oleaginous micro-organisms. Biochem Soc Trans 30:1047–1050.  https://doi.org/10.1042/bst0301047 CrossRefPubMedPubMedCentralGoogle Scholar
  27. 27.
    Ratledge C (2014) The role of malic enzyme as the provider of NADPH in oleaginous microorganisms: a reappraisal and unsolved problems. Biotechnol Lett 36:1557–1568CrossRefGoogle Scholar
  28. 28.
    Shen H, Gong Z, Yang X, Jin G, Bai F, Zhao ZK (2013) Kinetics of continuous cultivation of the oleaginous yeast Rhodosporidium toruloides. J Biotechnol 168:85–89CrossRefPubMedCentralGoogle Scholar
  29. 29.
    Shields-Menard SA, Amirsadeghi M, Sukhbaatar B, Revellame E, Hernandez R, Donaldson JR, French WT (2015) Lipid accumulation by Rhodococcus rhodochrous grown on glucose. J Ind Microbiol Biotechnol 42:693–699CrossRefPubMedCentralGoogle Scholar
  30. 30.
    Wen Z-Y, Chen F (2001) A perfusion–cell bleeding culture strategy for enhancing the productivity of eicosapentaenoic acid by Nitzschia laevis. Appl Microbiol Biotechnol 57:316–322CrossRefPubMedCentralGoogle Scholar
  31. 31.
    Wiebe MG, Koivuranta K, Penttilä M, Ruohonen L (2012) Lipid production in batch and fed-batch cultures of Rhodosporidium toruloides from 5 and 6 carbon carbohydrates. BMC Biotechnol 12:26CrossRefPubMedCentralGoogle Scholar
  32. 32.
    Ykema A, Verbree EC, Kater MM, Smit H (1988) Optimization of lipid production in the oleaginous yeast Apiotrichum curvatum in wheypermeate. Appl Microbiol Biotechnol 29:211–218.  https://doi.org/10.1007/bf00939309 CrossRefGoogle Scholar
  33. 33.
    Yusuf N, Kamarudin S, Yaakub Z (2011) Overview on the current trends in biodiesel production. Energy Convers Manage 52:2741–2751CrossRefGoogle Scholar

Copyright information

© Society for Industrial Microbiology and Biotechnology 2018

Authors and Affiliations

  1. 1.School of Chemical EngineeringNorthwest UniversityXi’anChina
  2. 2.School of Chemical Engineering and TechnologyXi’an Jiaotong UniversityXi’anChina
  3. 3.College of Biological and Chemical Engineering, Ningbo Institute of TechnologyZhejiang UniversityNingboChina
  4. 4.Department of BioengineeringUniversity of Massachusetts DartmouthNorth DartmouthUSA
  5. 5.Department of Chemical and Biomolecular EngineeringKAISTDaejeonKorea

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