Enhanced N-acetyl-d-neuraminic production from glycerol and N-acetyl-d-glucosamine by metabolically engineered Escherichia coli with a two-stage pH-shift control strategy

  • De-qiang Zhu
  • Jian-rong Wu
  • Xiao-bei ZhanEmail author
  • Li Zhu
  • Yun Jiang
Biocatalysis - Short Communication


Typical N-acetyl-d-neuraminic acid (Neu5Ac) production uses N-acetyl-d-glucosamine (GlcNAc) and excess pyruvate as substrates in the enzymatic or whole-cell biocatalysis process. In a previous study, a Neu5Ac-producing biocatalytic process via engineered Escherichia coli SA-05/pDTrc-AB/pCDF-pck-ppsA was constructed without exogenous pyruvate. In this study, glycerol was found to be a good energy source compared with glucose for the catalytic system with resting cells, and Neu5Ac production increased to 13.97 ± 0.27 g L−1. In addition, a two-stage pH shift strategy was carried out, and the Neu5Ac yield was improved to 14.61 ± 0.31 g L−1. The GlcNAc concentration for Neu5Ac production was optimized. Finally, an integrated strategy was developed for Neu5Ac production, and the Neu5Ac yield reached as high as 18.17 ± 0.27 g L−1. These results provide a new biocatalysis technology for Neu5Ac production without exogenous pyruvate.


Biocatalytic process Fed-batch N-Acetyl-d-neuraminic acid Glycerol Energy source 



This research was supported by the National Natural Science Foundation of China (no. 31171640), the Program of Introducing Talents of Discipline to Universities (111-2-06), the Fundamental Research Funds for the Central Universities (JUSRP51504, JUSRP51632A), and the National first-class discipline program of light industry technology and engineering (LITE2018-17).

Supplementary material

10295_2018_2132_MOESM1_ESM.docx (110 kb)
Supplementary material 1 (DOCX 111 KB)


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Copyright information

© Society for Industrial Microbiology and Biotechnology 2019

Authors and Affiliations

  1. 1.Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of BiotechnologyJiangnan UniversityWuxiChina
  2. 2.State Key Laboratory of Biobased Material and Green PapermakingQilu University of Technology, Shandong Academy of SciencesJinanChina
  3. 3.Jiangsu Rayguang Biotech Co. Ltd.WuxiChina

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