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The osnR gene of Corynebacterium glutamicum plays a negative regulatory role in oxidative stress responses

  • Haeri Jeong
  • Younhee Kim
  • Heung-Shick LeeEmail author
Genetics and Molecular Biology of Industrial Organisms - Original Paper
  • 150 Downloads

Abstract

Among the Corynebacterium glutamicum ORFs that have been implicated in stress responses, we chose ORF cg3230, designated osnR, and analyzed it further. Unlike the osnR-deleted strain (ΔosnR), the osnR-overexpressing strain (P180-osnR) developed growth defects and increased sensitivity to various oxidants including H2O2. Transcription in the P180-osnR strain of genes such as sodA (superoxide dismutase), ftn (ferritin biosynthesis), and ahpD (alkyl hydroperoxide reductase; cg2674), which are involved in the detoxification of reactive oxygen species, was only 40% that of the wild type. However, transcription of katA, encoding H2O2-detoxifying catalase, was unchanged in this strain. Genes such as trxB (thioredoxin reductase) and mtr (mycothiol disulfide reductase), which play roles in redox homeostasis, also showed decreased transcription in the strain. 2D-PAGE analysis indicated that genes involved in redox reactions were considerably affected by osnR overexpression. The NADPH/NADP+ ratio of the P180-osnR strain (1.35) was higher than that of the wild-type stain (0.78). Collectively, the phenotypes of the ΔosnR and P180-osnR strains suggest a global regulatory role as well as a negative role for the gene in stress responses, particularly in katA-independent oxidative stress responses.

Keywords

Corynebacterium glutamicum osnR Oxidative stress Reactive oxygen species 

Notes

Acknowledgements

This work was supported by the Basic Science Research Program, through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A3A03916638).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

10295_2018_2126_MOESM1_ESM.docx (1.2 mb)
Supplementary material 1 (DOCX 1271 kb)

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Copyright information

© Society for Industrial Microbiology and Biotechnology 2019

Authors and Affiliations

  1. 1.Department of Biotechnology and BioinformaticsKorea UniversitySejongSouth Korea
  2. 2.Department of Korean MedicineSemyung UniversityJecheonSouth Korea

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