High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus

  • Aureliano Claret da Cunha
  • Lorena Soares Gomes
  • Fernanda Godoy-Santos
  • Fábio Faria-Oliveira
  • Janaína Aparecida Teixeira
  • Geraldo Magela Santos Sampaio
  • Maria José Magalhães Trópia
  • Ieso Miranda Castro
  • Cândida Lucas
  • Rogelio Lopes BrandãoEmail author
Genetics and Molecular Biology of Industrial Organisms - Original Paper


Wickerhamomyces anomalus strain LBCM1105 was originally isolated from the wort of cachaça (the Brazilian fermented sugarcane juice-derived Brazilian spirit) and has been shown to grow exceptionally well at high amounts of glycerol. This paramount residue from the biodiesel industry is a promising cheap carbon source for yeast biotechnology. The assessment of the physiological traits underlying the W. anomalus glycerol consumption ability in opposition to Saccharomyces cerevisiae is presented. A new WaStl1 concentrative glycerol-H+ symporter with twice the affinity of S. cerevisiae was identified. As in this yeast, WaSTL1 is repressed by glucose and derepressed/induced by glycerol but much more highly expressed. Moreover, LBCM1105 aerobically growing on glycerol was found to produce ethanol, providing a redox escape to compensate the redox imbalance at the level of cyanide-resistant respiration (CRR) and glycerol 3P shuttle. This work is critical for understanding the utilization of glycerol by non-Saccharomyces yeasts being indispensable to consider their industrial application feeding on biodiesel residue.


Wickerhamomyces anomalus Glycerol metabolism Cachaça Biotechnological applications Glycerol transport STL1 



This work was supported by grants from Fundação de Capacitação de Pessoal de Nível Superior from the Ministry of Education—CAPES/Brazil (PNPD 2755/2011; PCF-PVE 021/2012), from FEDER through POFC-COMPETE and by FCT through strategic funding (UID/BIA/04050/2013), from Universidade Federal de Ouro Preto, and a research fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq (Brazil) Process 304815/2012 (research grant) and Process 305135/2015-5 (research fellowship to R.L.B.). C.L. is supported by the strategic program UID/BIA/04050/2013 [POCI-01-0145-FEDER-007569] funded by national funds through the FCT I.P. and by the ERDF through the COMPETE2020—Programa Operacional Competitividade e Internacionalização (POCI).The AUXPE-PVES 1801/2012 (Process 23038.015294/2016-18) from Brazilian Government supported a grant of Visiting Professor to C.L. and a research fellowships to A.C.C. and to F.F.O.

Compliance with ethical standards

Conflict of interest

Authors wish to confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome.


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© Society for Industrial Microbiology and Biotechnology 2019

Authors and Affiliations

  • Aureliano Claret da Cunha
    • 1
  • Lorena Soares Gomes
    • 1
  • Fernanda Godoy-Santos
    • 1
  • Fábio Faria-Oliveira
    • 1
  • Janaína Aparecida Teixeira
    • 1
  • Geraldo Magela Santos Sampaio
    • 1
  • Maria José Magalhães Trópia
    • 1
  • Ieso Miranda Castro
    • 1
  • Cândida Lucas
    • 2
  • Rogelio Lopes Brandão
    • 1
    Email author
  1. 1.Laboratório de Biologia Celular e MolecularNUPEB, Universidade Federal de Ouro PretoOuro PretoBrazil
  2. 2.Instituto de Ciência e Inovação em Bio-Sustentabilidade (IB-S)/Centro de Biologia Molecular e Ambiental (CBMA)Universidade do MinhoBragaPortugal

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