RETRACTED ARTICLE: Comparative analysis of the Corynebacterium glutamicum transcriptome in response to changes in dissolved oxygen levels

  • Xiuxia Liu
  • Sun Yang
  • Fen Wang
  • Xiaofeng Dai
  • Yankun YangEmail author
  • Zhonghu BaiEmail author
Fermentation, Cell Culture and Bioengineering - Original Paper


The dissolved oxygen (DO) level of a culture of Corynebacterium glutamicum (C. glutamicum) in a bioreactor has a significant impact on the cellular redox potential and the distribution of energy and metabolites. In this study, to gain a deeper understanding of the effects of DO on the metabolism of C. glutamicum, we sought to systematically explore the influence of different DO concentrations on genetic regulation and metabolism through transcriptomic analysis. The results revealed that after 20 h of fermentation, oxygen limitation enhanced the glucose metabolism, pyruvate metabolism and carbon overflow, and restricted NAD+ availability. A high oxygen supply enhanced the TCA cycle and reduced glyoxylate metabolism. Several key genes involved in response of C. glutamicum to different oxygen concentrations were examined, which provided suggestions for target site modifications in developing optimized oxygen supply strategies. These data provided new insights into the relationship between oxygen supply and metabolism of C. glutamicum.


Corynebacterium glutamicum Dissolved oxygen Transcriptome Metabolism Bioprocess 



This study was funded by the National Basic Research Program of China (973 Program) (Grant Number 2013CB733602), the Fundamental Research Funds for the Central Universities (Grant Number JUSRP51401A), the National Natural Science Foundation of China (Grant Number 31570034), and the Natural Science Foundation of Jiangsu Province (Grant Number BK20150148).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10295_2016_1854_MOESM1_ESM.pdf (1.9 mb)
Supplementary material 1 (PDF 1951 kb)


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Copyright information

© Society for Industrial Microbiology and Biotechnology 2016

Authors and Affiliations

  1. 1.National Engineering Laboratory for Cereal Fermentation TechnologyJiangnan UniversityWuxiChina
  2. 2.The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of BiotechnologyJiangnan UniversityWuxiChina
  3. 3.The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of BiotechnologyJiangnan UniversityWuxiChina

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