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Effect of vasopressin as a local anesthetic in mice


We sought to elucidate how the local administration of mepivacaine hydrochloride and vasopressin via the tail affects the peripheral blood flow volume, tissue dynamics, and mepivacaine’s anesthetic effect in mice. Two-hundred and twenty-six male ICR mice were used in this study. Blood flow was measured after administering mepivacaine alone or mepivacaine with either 0.03, 0.3, or 3.0 U/mL vasopressin or 10 µg/mL epinephrine via the tail tissue. The tail tissue and blood dynamics were measured using 3H-labeled mepivacaine hydrochloride with vasopressin or epinephrine. The compound nerve action potential (CNAP) was measured to clarify the anesthetic effect after administering mepivacaine with 0.3 U/mL vasopressin. The statistical methods employed were Steel–Dwass test, Mann–Whitney U test, Dunnett’s test, and Tukey test. P < 0.05 indicated statistical significance. The results revealed that the local administration of ≥ 0.03 U/mL vasopressin reduced local blood flow and prolonged 3H-M localization in the tail tissue in a concentration-dependent manner. Addition of 0.3 U/mL vasopressin enhanced and prolonged the anesthetic effect of mepivacaine. The findings suggest that adding vasopressin to a local anesthetic regimen may be effective, and thus it could be applied as a vasoconstrictor.

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We would like to thank Editage for English language editing.


This study was funded by JSPS KAKENHI Grant Number JP19K19249.

Author information

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by KK, SH and KS. The first draft of the manuscript was written by KK and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Correspondence to Keiko Katagiri.

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The authors declare that they have no conflict of interest.

Ethical approval

This study was approved by the Animal Use and Care Committee of the Nippon Dental University School of Life Dentistry (Approval Number: 17-12) and was conducted in accordance with the committee’s guidelines.

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Katagiri, K., Hashimoto, S. & Sunda, K. Effect of vasopressin as a local anesthetic in mice. Odontology (2020). https://doi.org/10.1007/s10266-020-00491-2

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  • Vasopressin
  • Mepivacaine
  • Epinephrine
  • Blood vessel
  • Vasoconstriction