, Volume 107, Issue 1, pp 23–28 | Cite as

Dentin bonding and physicochemical properties of adhesives incorporated with epigallocatechin-3-gallate

  • Francisco Adeilson Alves de Macedo
  • Nayara O. Souza
  • Marcelo Victor Sidou Lemos
  • Diego Martins De-Paula
  • Sergio Lima Santiago
  • Victor Pinheiro FeitosaEmail author
Original Article


The aim was to assess dentin adhesion and physicochemical properties of experimental etch-and-rinse and self-etch adhesives doped with epigallocatechin-3-gallate (EGCG), a well-known collagen crosslinker obtained from green tea (Camellia sinensis). Experimental adhesives were prepared without (0 wt%), with 0.1 or 0.5 wt% EGCG addition. Their degree of conversion was surveyed by FTIR, and bar-shaped specimens were tested to obtain flexural strength and modulus initially and after 1-week ethanol storage. Extracted human molars were prepared, bonded, and cut into resin–dentin sticks for microtensile bond strength test, which was conducted after 24 h or 6-month water storage. Statistical analyses were performed with two-way ANOVA and Tukey’s test (p < 0.05). Degree of conversion outcomes depicted a significant polymerization reduction by the addition of EGCG in self-etch adhesive in both concentrations. However, only 0.1% reduced the conversion of etch-and-rinse adhesive. Flexural modulus and strength were significantly diminished (p < 0.05) by the addition of both concentrations of EGCG for the two model adhesives. Dentin-bond strength was reduced after aging with the addition of EGCG to self-etch adhesive. Nevertheless, 0.5% EGCG increased the bond strength of etch-and-rinse adhesive after aging, conversely to the significant reduction for EGCG-free control adhesive. In conclusion, EGCG at 0.5% provides optimal improvements on dentin bonding without altering final polymerization of a model etch-and-rinse adhesive.


Bond strength Dental adhesive Green tea Elastic modulus Dentin 



This investigation was partially supported by grant CNPQ/Universal 2014 Project 457931/2014-0 (PI VPF). We also thank Capes and CNPQ Brazilian agencies for the scholarships provided. We are grateful to Essetech Inc. for the donation of monomers and further reagents.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© The Society of The Nippon Dental University 2018

Authors and Affiliations

  • Francisco Adeilson Alves de Macedo
    • 1
  • Nayara O. Souza
    • 1
  • Marcelo Victor Sidou Lemos
    • 1
  • Diego Martins De-Paula
    • 1
    • 2
  • Sergio Lima Santiago
    • 1
  • Victor Pinheiro Feitosa
    • 1
    • 2
    Email author
  1. 1.Department of Restorative Dentistry, Dental SchoolFederal University of CearaFortalezaBrazil
  2. 2.Paulo Picanço School of DentistryFortalezaBrazil

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