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Odontology

pp 1–8 | Cite as

Structural and biomechanical changes to dentin extracellular matrix following chemical removal of proteoglycans

  • Ana Paula Farina
  • Cristina M. P. Vidal
  • Doglas Cecchin
  • Thaiane R. Aguiar
  • Ana K. Bedran-RussoEmail author
Original Article
  • 34 Downloads

Abstract

Proteoglycans are biomacromolecules with significant biomineralization and structural roles in the dentin extracellular matrix. This study comprehensively assessed the mechanical properties and morphology of the dentin extracellular matrix following chemical removal of proteoglycans to elucidate the structural roles of proteoglycans in dentin. Dentin extracellular matrix was prepared from extracted teeth after complete tissue demineralization. Chemical removal of proteoglycans was carried-out using guanidine hydrochloride for up to 10 days. The removal of proteoglycans was determined by dimethylmethylene blue colorimetric assay and histological staining analyses using transmission electron microscopy and optical microscopy. The modulus of elasticity of dentin matrix was determined by a 3-point bending test method. Partial removal of proteoglycans induced significant modifications to the dentin matrix, particularly to type I collagen. Removal of proteoglycans significantly decreased the modulus of elasticity of dentin extracellular matrix (p < 0.0001). In conclusion, the subtle disruption of proteoglycans induces pronounced changes to the collagen network packing and the bulk modulus of elasticity of dentin matrix.

Keywords

Proteoglycans Collagen Dentin Modulus of elasticity Histology 

Notes

Acknowledgements

The study was supported by the National Institute of Health—NIH/NIDCR (#DE021040) and CAPES Foundation-Brazil (#BEX 17764/12-2). The authors would like to thank Ariene Leme-Kraus for the support with the statistical analysis.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© The Society of The Nippon Dental University 2019

Authors and Affiliations

  • Ana Paula Farina
    • 1
    • 2
  • Cristina M. P. Vidal
    • 1
    • 3
  • Doglas Cecchin
    • 1
    • 2
  • Thaiane R. Aguiar
    • 1
    • 4
  • Ana K. Bedran-Russo
    • 1
    Email author
  1. 1.Department of Restorative Dentistry, College of DentistryUniversity of Illinois at ChicagoChicagoUSA
  2. 2.Department of Restorative Dentistry, Passo Fundo Dental SchoolUniversity of Passo Fundo, UPFPasso FundoBrazil
  3. 3.Department of Operative Dentistry, College of DentistryUniversity of IowaIowa CityUSA
  4. 4.Department of Clinical Dentistry, School of DentistryFederal University of BahiaSalvadorBrazil

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