Lag3+ regulatory T lymphocytes in critical carotid artery stenosis

  • F. Del PortoEmail author
  • N. Cifani
  • M. Proietta
  • T. Dezi
  • L. Tritapepe
  • S. Raffa
  • A. Micaloni
  • M. Taurino
Original Article


The aim of this study was to evaluate CD25+ and Lag3+ T regulatory subpopulations in patients with critical carotid artery stenosis (CAS) and Stanford-A acute aortic dissection (AAD). CD25+ and Lag3+ were measured in 36 patients affected by CAS and 24 patients with Stanford type A AAD. Based on neurological symptoms, patients affected by CAS were further divided in 25 asymptomatic (CAS-A) and 11 symptomatic (CAS-S) subjects. Twenty-five patients with traditional cardiovascular risk factors (RF), matched for age and sex, were used as control group. Interleukin (IL)-10, IL-6 and transforming growth factor-β-levels were also measured. CD25+ T cells were significantly increased in CAS-S versus CAS-A (p > 0.05), AAD (p > 0.05) and RF (p > 0.05). Moreover, a significant increase in Lag3+ Tregs was observed in CAS e CAS-S versus AAD (p < 0.05) and RF (p < 0.05), whereas no significant difference was observed between CAS-S and CAS-A. IL-6 was higher in AAD compared to the other groups. Patients with neurological symptoms display a peculiar expansion of CD25+ T cells, strongly confirming a relationship between ischemic brain damage and this regulatory subpopulation, whereas Lag3+ Tregs early distinguish CAS from AAD and probably exert protective actions against aortic wall rupture throughout their anti-inflammatory functions.


Regulatory T cells Carotid artery stenosis Acute aortic dissection 


Compliance with ethical standards

Conflict of interest

The authors have no actual or potential conflict of interest to declare, including any financial, personal or other relationships with other people or organizations within 3 years of beginning the submitted work that could inappropriately influence, or be perceived to influence, their work.

Ethical approval

The study was performed according to the principles of the Declaration of Helsinki and was approved by the Ethics Committee of the Faculty of Medicine.

Informed consent

Written informed consent was obtained from each patient or from an authorized family member.


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Dipartimento di Medicina Clinica e Molecolare, Facoltà di Medicina e Psicologia, Ospedale Sant’Andrea, UOC Medicina InternaSapienza Università di RomaRomeItaly
  2. 2.Dipartimento di Medicina Clinica e Molecolare, Facoltà di Medicina e Psicologia, Ospedale Sant’Andrea, UOC Chirurgia VascolareSapienza Università di RomaRomeItaly
  3. 3.Dipartimento di Scienze Anestesiologiche, Facoltà di Medicina e Odontoiatria, Medicina Critica e Terapia del Dolore, Policlinico Umberto ISapienza Università di RomaRomeItaly
  4. 4.Dipartimento di Medicina Clinica e Molecolare, Facoltà di Medicina e Psicologia, Ospedale Sant’Andrea, UOC Diagnostica CellulareSapienza Università di RomaRomeItaly

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