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Clinical and Experimental Medicine

, Volume 19, Issue 3, pp 377–384 | Cite as

Expression of tetraspanins NET-6 and CD151 in breast cancer as a potential tumor biomarker

  • Liejun Jiang
  • Xiliu Zhang
  • Joseph Geradts
  • Qiang Wei
  • Steven Hochwald
  • Hui Xu
  • Huayi HuangEmail author
Original Article
  • 118 Downloads

Abstract

Tetraspanins have been implicated in multiple biological functions including protein networking and cell signaling. NET-6 (TSPAN 13) has been demonstrated to be a tumor suppressor gene in breast cancer, while CD151 is more likely to act as an oncogene. However, the biological function of both proteins is still inconclusive. Immunohistochemistry was used to analyze the expression of NET-6 and CD151 proteins in breast tumors and benign epithelial cells. The cellular expression of both markers was correlated with HER2, ER, and PR status as well as tumor grade, Ki-67 scores, invasion, and metastasis. Expression of NET-6 and CD151 was variable both in tumors and in benign epithelial cells. Expression of NET-6 and CD151 was stronger in tumors than in benign epithelial cells. The expression of NET-6 was also stronger in HER2-negative, low-grade, lymphovascular invasion-negative, and non-metastatic breast tumors. There was no correlation between NET-6 expression and ER, or PR, or triple-negative status. There was no correlation between CD151 expression and HER2, ER, PR, or triple-negative status, tumor grade, or Ki-67 scores, invasion, and metastasis. The expression of tetraspanins NET-6 and CD151 may indicate an alteration of their biological function during neoplastic transformation. NET-6 expression in tumors might be a potential marker indicating the outcome of breast cancer.

Keywords

Tetraspanin NET-6 (TSPAN 13) CD151 Breast cancer Metastasis 

Notes

Acknowledgements

This study was funded by grants from the Guangxi Bureau of Science and Technology, No. 1598013-3 (PI: Huayi Huang) and No. 2017GXNSFAA198043 (PI: Huayi Huang) and a grant from The Health and Family Planning Commission of Guangxi Zhuang Autonomous Region, No. Z2015353 (PI: Huayi Huang).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The study was approved by the Institute Review Boards of Guangzhou Huayin Medical Laboratory and the People’s Hospital of Guangxi Zhuang Autonomous Region for the use of human materials.

Informed consent

This study was waived for the patients’ informed consent from the above-mentioned institutions because the study was a retrospective with pathology archived tissues.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of Laboratory MedicineThe People’s Hospital of Guangxi Zhuang Autonomous RegionNanningChina
  2. 2.Department of PathologyThe First Affiliated Hospital of Guangxi Traditional Chinese MedicineNanningChina
  3. 3.Department of Population SciencesCity of Hope National Medical CenterDuarteUSA
  4. 4.Department of PathologyThe People’s Hospital of Guangxi Zhuang Autonomous RegionNanningChina
  5. 5.Department of Surgical OncologyRoswell Park Comprehensive Cancer CenterBuffaloUSA
  6. 6.Department of PathologyHuayin Medical LaboratoryGuangzhouChina

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