Tetraspanins have been implicated in multiple biological functions including protein networking and cell signaling. NET-6 (TSPAN 13) has been demonstrated to be a tumor suppressor gene in breast cancer, while CD151 is more likely to act as an oncogene. However, the biological function of both proteins is still inconclusive. Immunohistochemistry was used to analyze the expression of NET-6 and CD151 proteins in breast tumors and benign epithelial cells. The cellular expression of both markers was correlated with HER2, ER, and PR status as well as tumor grade, Ki-67 scores, invasion, and metastasis. Expression of NET-6 and CD151 was variable both in tumors and in benign epithelial cells. Expression of NET-6 and CD151 was stronger in tumors than in benign epithelial cells. The expression of NET-6 was also stronger in HER2-negative, low-grade, lymphovascular invasion-negative, and non-metastatic breast tumors. There was no correlation between NET-6 expression and ER, or PR, or triple-negative status. There was no correlation between CD151 expression and HER2, ER, PR, or triple-negative status, tumor grade, or Ki-67 scores, invasion, and metastasis. The expression of tetraspanins NET-6 and CD151 may indicate an alteration of their biological function during neoplastic transformation. NET-6 expression in tumors might be a potential marker indicating the outcome of breast cancer.
Tetraspanin NET-6 (TSPAN 13) CD151 Breast cancer Metastasis
This is a preview of subscription content, log in to check access.
This study was funded by grants from the Guangxi Bureau of Science and Technology, No. 1598013-3 (PI: Huayi Huang) and No. 2017GXNSFAA198043 (PI: Huayi Huang) and a grant from The Health and Family Planning Commission of Guangxi Zhuang Autonomous Region, No. Z2015353 (PI: Huayi Huang).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
The study was approved by the Institute Review Boards of Guangzhou Huayin Medical Laboratory and the People’s Hospital of Guangxi Zhuang Autonomous Region for the use of human materials.
This study was waived for the patients’ informed consent from the above-mentioned institutions because the study was a retrospective with pathology archived tissues.
Kwon HJ, Choi JE, Kang SH, Son Y, Bae YK. Prognostic significance of CD9 expression differs between tumour cells and stromal immune cells, and depends on the molecular subtype of the invasive breast carcinoma. Histopathology. 2017;70:1155–65. https://doi.org/10.1111/his.13184.CrossRefGoogle Scholar
Romanska HM, Potemski P, Kusinska R, Kopczynski J, Sadej R, Kordek R. Expression of CD151/Tspan24 and integrin alpha 3 complex in aid of prognostication of HER2-negative high-grade ductal carcinoma in situ. Int J Clin Exp Pathol. 2015;8:9471–8.Google Scholar
Fisher OM, Levert-Mignon AJ, Lehane CW, et al. CD151 gene and protein expression provides independent prognostic information for patients with adenocarcinoma of the esophagus and gastroesophageal junction treated by esophagectomy. Ann Surg Oncol. 2016;23:746–54. https://doi.org/10.1245/s10434-016-5504-9.CrossRefGoogle Scholar
Wadkin J, Patten DA, Kamarajah SK, et al. CD151 supports VCAM-1-mediated lymphocyte adhesion to liver endothelium and is upregulated in chronic liver disease and hepatocellular carcinoma. Am J Physiol Gastrointest Liver Physiol. 2017;313:G138–49. https://doi.org/10.1152/ajpgi.00411.2016.CrossRefGoogle Scholar