, Volume 20, Issue 2, pp 215–224 | Cite as

Variable relationships between the hydrophobic fraction of dissolved organic matter and metals in Scottish freshwater before the estuarine mixing zone

  • Hajime Sato
  • Morimaru Kida
  • Satoko Yamano
  • Haruka Sonoda
  • Nobuhide FujitakeEmail author
Research paper


The organic-Fe association in Scottish freshwater rivers has received little attention compared with in the estuarine mixing zone. We collected 201 water samples from rivers and lakes in Scotland across different sampling years and seasons. Relationships among the hydrophobic (HPO) fraction of dissolved organic matter (DOM), specific UV absorbance (SUVA254), and dissolved metals (Al and Fe) were examined to better understand their co-transportation in Scottish waters. The average DOM, HPO fraction, Fe, and Al concentrations for all the samples co-varied and were lower during winter than during summer. There was a strong positive correlation between DOM and HPO fraction concentrations (R2 = 0.99, p < 0.0001). A significant positive correlation was also found between the HPO fraction and Fe and Al concentrations. The regression slope indicating the overall relationships between the HPO fraction and Fe concentrations differed by as much as 12 times depending on both the sampling period and the river. These slope differences were not significantly determined by the chemical structures of DOM, SUVA254, or Al and Cu concentrations. These results suggest that the Fe transport capacities vary among the Scottish rivers because of other factors such as seasonal effects (temperature and the level of water table) and a suspended solid concentration in the water column.


Complex DOM Iron Peat SUVA254 



This work was supported by JSPS KAKENHI Grant Number JP15H02805.

Compliance with ethical standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

10201_2018_569_MOESM1_ESM.docx (898 kb)
Supplementary material 1 (DOCX 898 kb)


  1. Blazevic A, Orlowska E, Kandioller W et al (2016) Photoreduction of terrigenous Fe–humic substances leads to bioavailable iron in oceans. Angew Chem Int Ed 55:6417–6422. CrossRefGoogle Scholar
  2. Butman D, Raymond PA, Butler K, Aiken G (2012) Relationships between Δ14C and the molecular quality of dissolved organic carbon in rivers draining to the coast from the conterminous United States. Glob Biogeochem Cycles 26:1–15. CrossRefGoogle Scholar
  3. Dai MH, Martin JM (1995) First data on trace metal level and behaviour in two major Arctic river-estuarine systems (Ob and Yenisey) and in the adjacent Kara Sea, Russia. Earth Planet Sci Lett 131:127–141. CrossRefGoogle Scholar
  4. Gustafsson JP (2001) Modeling the acid–base properties and metal complexation of humic substances with the Stockholm Humic Model. J Colloid Interface Sci 244:102–112. CrossRefGoogle Scholar
  5. Hanley KW, Wollheim WM, Salisbury J et al (2013) Controls on dissolved organic carbon quantity and chemical character in temperate rivers of North America. Global Biogeochem Cycles 27:492–504. CrossRefGoogle Scholar
  6. Imai A, Fukushima T, Matsushige K, Kim YH (2001) Fractionation and characterization of dissolved organic matter in a shallow eutrophic lake, its inflowing rivers, and other organic matter sources. Water Res 35:4019–4028. CrossRefGoogle Scholar
  7. Kida M, Maki K, Takata A et al (2015) Quantitative monitoring of aquatic humic substances in Lake Biwa, Japan, using the DAX-8 batch method based on carbon concentrations. Org Geochem 83–84:153–157. CrossRefGoogle Scholar
  8. Kida M, Fujitake N, Suchewaboripont V et al (2018a) Contribution of humic substances to dissolved organic matter optical properties and iron mobilization. Aquat Sci 80:26. CrossRefGoogle Scholar
  9. Kida M, Myangan O, Oyuntsetseg B et al (2018b) Dissolved organic matter distribution and its association with colloidal aluminum and iron in the Selenga River Basin from Ulaanbaatar to Lake Baikal. Environ Sci Pollut Res. Google Scholar
  10. Kikuchi T, Fujii M, Terao K et al (2017) Correlations between aromaticity of dissolved organic matter and trace metal concentrations in natural and effluent waters: a case study in the Sagami River Basin, Japan. Sci Total Environ 576:36–45. CrossRefGoogle Scholar
  11. Krachler R, Jirsa F, Ayromlou S (2005) Factors influencing the dissolved iron input by river water to the open ocean. Biogeosciences Discuss 2:311–315. CrossRefGoogle Scholar
  12. Krachler R, Krachler RF, von der Kammer F et al (2010) Relevance of peat-draining rivers for the riverine input of dissolved iron into the ocean. Sci Total Environ 408:2402–2408. CrossRefGoogle Scholar
  13. Krachler R, Krachler RF, Wallner G et al (2015) River-derived humic substances as iron chelators in seawater. Mar Chem 174:85–93. CrossRefPubMedCentralGoogle Scholar
  14. Krachler R, Krachler RF, Wallner G et al (2016) Sphagnum-dominated bog systems are highly effective yet variable sources of bio-available iron to marine waters. Sci Total Environ 556:53–62. CrossRefGoogle Scholar
  15. Laglera LM, van den Berg CMG (2009) Evidence for geochemical control of iron by humic substances in seawater. Limnol Oceanogr 54(2):610–619. CrossRefGoogle Scholar
  16. Martin JM, Windom H (1991) Present and future role of ocean margins in regulating marine biogeochemical cycles of trace elements. In: Mantoura R, Martin J, Wollast R (eds) Ocean margine processes in global change. Wiley, New York, pp 45–67Google Scholar
  17. Muller FLL, Tankéré-Muller SPC (2012) Seasonal variations in surface water chemistry at disturbed and pristine peatland sites in the Flow Country of northern Scotland. Sci Total Environ 435–436:351–362. CrossRefGoogle Scholar
  18. Muller FLL, Chang KC, Lee CL, Chapman SJ (2015) Effects of temperature, rainfall and conifer felling practices on the surface water chemistry of northern peatlands. Biogeochemistry 126:343–362. CrossRefGoogle Scholar
  19. Myangan O, Kawahigashi M, Oyuntsetseg B, Fujitake N (2017) Impact of land uses on heavy metal distribution in the Selenga River system in Mongolia. Environ Earth Sci 76:346–360. CrossRefGoogle Scholar
  20. Poulin BA, Ryan JN, Aiken GR (2014) Effects of iron on optical properties of dissolved organic matter. Environ Sci Technol 48:10098–10106. CrossRefGoogle Scholar
  21. Schellekens J, Buurman P, Kalbitz K et al (2017) Molecular features of humic acids and fulvic fcids from contrasting environments. Environ Sci Technol 51:1330–1339. CrossRefGoogle Scholar
  22. Sholkovitz E, Boyle E, Price N (1978) The removal of dissolved humic acids and iron during estuarine mixing. Earth Planet Sci Lett 40:130–136. CrossRefGoogle Scholar
  23. Spencer RG, Butler KD, Aiken GR (2012) Dissolved organic carbon and chromophoric dissolved organic matter properties of rivers in the USA. J Geophys Res 117:1–14. CrossRefGoogle Scholar
  24. Taylor NS, Kirwan JA, Yan ND et al (2015) Metabolomics confirms that dissolved organic carbon mitigates copper toxicity. Environ Toxicol Chem 35:635–644. CrossRefGoogle Scholar
  25. Tipping E (1998) Humic Ion-Binding Model VI: an improved description of the interactions of protons and metal ions with humic substances. Aquat Geochem 4:3–48. CrossRefGoogle Scholar
  26. Tipping E (2002) Cation binding by humic substances. Cambridge University Press, CambridgeCrossRefGoogle Scholar
  27. Tsuda K, Mori H, Asakawa D et al (2010) Characterization and grouping of aquatic fulvic acids isolated from clear-water rivers and lakes in Japan. Water Res 44:3837–3846. CrossRefGoogle Scholar
  28. Tsuda K, Takata A, Shirai H et al (2012) A method for quantitative analysis of aquatic humic substances in clear water based on carbon concentration. Anal Sci 28:1017–1020. CrossRefGoogle Scholar
  29. Tsuda K, Kida M, Aso S et al (2016) Determination of aquatic humic substances in Japanese lakes and wetlands by the carbon concentration-based resin isolation technique. Limnology 17:1–6. CrossRefGoogle Scholar
  30. Watanabe A, Moroi K, Sato H et al (2012) Contributions of humic substances to the dissolved organic carbon pool in wetlands from different climates. Chemosphere 88(10):1265–1268CrossRefGoogle Scholar
  31. Weishaar JL, Aiken GR, Bergamaschi BA et al (2003) Evaluation of specific ultraviolet absorbance as an indicator of the chemical composition and reactivity of dissolved organic carbon. Environ Sci Technol 37:4702–4708. CrossRefGoogle Scholar
  32. Zhou Z, Hua B, Cao X et al (2015) Chemical composition of dissolved organic matter from various sources as characterized by solid-state NMR. Aquat Sci 77:595–607. CrossRefGoogle Scholar

Copyright information

© The Japanese Society of Limnology 2019

Authors and Affiliations

  1. 1.Graduate School of Agricultural ScienceKobe UniversityKobeJapan

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