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Membrane proteomic analysis reveals the intestinal development is deteriorated by intrauterine growth restriction in piglets

Abstract

The alterations of the intestinal proteome were observed in intrauterine growth restriction (IUGR) piglets during early life by gel-based approaches. Nevertheless, how IUGR affects the intestinal membrane proteome during neonatal development remains unclear. Here, we applied the iTRAQ-based proteomics technology and biochemical analysis to investigate the impact of IUGR on the membrane proteome of the jejunal mucosa in the piglets. Three hundred sixty-one membrane proteins were screened by functional prediction. Among them, eight, five, and one differentially expressed membrane proteins were identified between IUGR and NBW piglets at day 0, day 7, and day 21 after birth, respectively. Differentially expressed membrane proteins (DEMPs) including F1SBL3, F1RRW8, F1S539, F1S2Z2, F1RIR2, F1RUF2 I3LP60, Q2EN79, and F1SIH8 were reduced while the relative abundance of I3L6A2, F1SCJ1, F1RI18, I3LRJ7, and F1RNN0 were increased in IUGR piglets than NBW piglets. From the aspects of function, F1RRW8, F1S539, F1S2Z2, and F1RIR2 are mainly associated with D2 dopamine receptor binding, transmembrane transport of small molecules, signal transduction, and translocation of GLUT4, respectively, and F1SIH8, I3LRJ7, and F1RNN0 are related to autophagy, metabolism of vitamins, and intracellular protein transport. Additionally, IUGR decreased the level of proteins (F1RRW8, Q2EN79, and F1RI18) that are involved in response to oxidative stress.

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Acknowledgments

We thank the Mianyang New-hope Livestock Farming Co. Ltd. in Sichuan Province, China, for their assistance in this study. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the iProX partner repository (Ma et al. 2019) with the dataset identifier PXD013315.

Funding

This work was supported by the National Natural Science Foundation of China (31630074), the Beijing Municipal Natural Science Foundation (S170001), the National Key Research and Development Program of China (2016YFD0500506, 2018YDF0501002), the 111 Project (B16044), and Jinxinnong Animal Science Developmental Foundation.

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Correspondence to Junjun Wang.

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All experiments were carried out with the approval of China Agricultural University Animal Care and Use Committee (CAU20170114–1, Beijing, China).

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The authors declared that they have no competing interests.

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Huang, S., Liu, C., Li, N. et al. Membrane proteomic analysis reveals the intestinal development is deteriorated by intrauterine growth restriction in piglets. Funct Integr Genomics 20, 277–291 (2020). https://doi.org/10.1007/s10142-019-00714-y

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Keywords

  • iTRAQ
  • Intrauterine growth restriction
  • Neonatal piglets
  • Membrane proteome
  • Intestine