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Functional & Integrative Genomics

, Volume 19, Issue 4, pp 541–554 | Cite as

Expressing class I wheat NHX (TaNHX2) gene in eggplant (Solanum melongena L.) improves plant performance under saline condition

  • Rajesh YarraEmail author
  • P. B. Kirti
Original Article

Abstract

Brinjal or eggplant (Solanum melongena L.) is an important solanaceous edible crop, and salt stress adversely affects its growth, development, and overall productivity. To cope with excess salinity, vacuolar Na+/H+ antiporters provide the best mechanism for ionic homeostasis in plants under salt stress. We generated transgenic eggplants by introducing wheat TaNHX2 gene that encodes a vacuolar Na+/H+ antiporter in to the eggplant genome via Agrobacterium-mediated transformation using pBin438 vector that harbors double35S:TaNHX2 to confer salinity tolerance. Polymerase chain reaction and southern hybridization confirmed the presence and integration of TaNHX2 gene in T1 transgenic plants. Southern positive transgenic eggplants showed varied levels of TaNHX2 transcripts as evident by RT-PCR and qRT-PCR. Stress-inducible expression of TaNHX2 significantly improved growth performance and Na+ and K+ contents from leaf and roots tissues of T2 transgenic eggplants under salt stress, compared to non-transformed plants. Furthermore, T2 transgenic eggplants displayed the stable leaf relative water content and chlorophyll content, proline accumulation, improved photosynthetic efficiency, transpiration rate, and stomatal conductivity than the non-transformed plants under salinity stress (200 mM NaCl). Data showed that the T2 transgenic lines revealed that reduction in MDA content, hydrogen peroxide, and oxygen radical production associated with the significant increase of antioxidant enzyme activity in transgenic eggplants than non-transformed plants under salt stress (200 mM NaCl). This study suggested that the TaNHX2 gene plays an important regulatory role in conferring salinity tolerance of transgenic eggplant and thus may serve as a useful candidate gene for improving salinity tolerance in other vegetable crops.

Keywords

TaNHX2 Solanum melongena Salt stress Vegetables 

Abbreviations

RT-PCR

Reverse transcription PCR

qRT-PCR

Quantitative real-time PCR

SOD

Superoxide dismutase

APX

Ascorbate peroxidase

GPX

Guaiacol peroxidase

GR

Glutathione reductase

MDA

Malondialdehyde

Notes

Acknowledgements

Authors acknowledge the Head, Department of Plant Sciences for access to the research facilities provided by DST-FIST, DBT-CREBB, and UGC-SAP to the Department of Plant Sciences, University of Hyderabad. The authors are thankful to Prof. Shouyi Chen and Prof. Jinsong Zhang, Institute of Genetics and Developmental Biology, CAS, Beijing for generous offer of the plasmid used in this study. We thank the anonymous reviewers for their valuable comments in improving the manuscript.

Author contributions

RY and PBK conceived the experiment. RY performed the experiment. RY and PBK analyzed the data. RY and PBK wrote the manuscript. All authors approved the final version of the manuscript.

Funding information

The Science and Engineering Research Board (SERB), Department of Science and Technology (DST), Govt. of India provided fund and fellowship under Young Scientist Scheme (SB/FT/LS-445/2012).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10142_2019_656_MOESM1_ESM.docx (234 kb)
ESM 1 (DOCX 234 kb)

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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Plant Sciences, School of Life SciencesUniversity of HyderabadHyderabadIndia

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