Marine Microorganisms for Biocatalysis: Selective Hydrolysis of Nitriles with a Salt-Resistant Strain of Meyerozyma guilliermondii
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A screening among marine yeasts was carried out for nitrile hydrolyzing activity. Meyerozyma guilliermondii LM2 (UBOCC-A-214008) was able to efficiently grow on benzonitrile and cyclohexanecarbonitrile (CECN) as sole nitrogen sources. A two-step one-pot method for obtaining cells of M. guilliermondii LM2 (UBOCC-A-214008) endowed with high nitrilase activity was established; the resulting whole cells converted different nitriles with high molar conversions and showed interesting enantioselectivity toward racemic substrates. Nitrilase from M. guilliermondii LM2 (UBOCC-A-214008) displayed high activity on aromatic substrates, but also arylaliphatic and aliphatic substrates were accepted. Salt-resistant M. guilliermondii LM2 (UBOCC-A-214008) was used in media with different salinity, being highly active up to 1.5 M NaCl concentration. Finally, hydrolysis of nitriles was efficiently performed using a bioprocess (yeast growth and biotransformation with resting cells) entirely carried out in seawater.
KeywordsMarine yeast Nitrilase Meyerozyma guilliermondii Seawater Biocatalysis
The authors acknowledge G. Burgaud (University of Brest) for kindly providing the yeast strains.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- De Vitis V, Guidi B, Contente ML Granato T, Conti P, Molinari F, Crotti E, Mapelli F, Borin S Daffonchio D, Romano D (2015) Marine microorganisms as source of stereoselective esterases and ketoreductases: kinetic resolution of a prostaglandin intermediate. Mar Biotechnol 17:144–152CrossRefGoogle Scholar
- Hoyle AJ, Bunch AW, Knowles CJ (1998) The nitrilases of Rhodococcus rhodochrous NCIMB 11216. Enzym Microb Technol. https://doi.org/10.1016/S0141-0229(98)00076-3
- Martínková L, Křen V (2010) Biotransformations with nitrilases. Curr Opin Chem Biol. https://doi.org/10.1016/j.cbpa.2009.11.018
- Mukherjee C, Zhu D, Biehl ER, Hua L (2006) Exploring the synthetic applicability of a cyanobacterium nitrilase as catalyst for nitrile hydrolysis. Eur J Org Chem. https://doi.org/10.1002/ejoc.200600699
- Petrícková A, Sosedov O, Baum S, Stolz A, Martínková L (2012) Influence of point mutations near the active site on the catalytic properties of fungal arylacetonitrilases from Aspergillus niger and Neurospora crassa. J Mol Catal B Enzym. https://doi.org/10.1016/j.molcatb.2012.01.005
- Serra I, Guidi B, Burgaud G, Contente ML, Ferraboschi P, Pinto A, Compagno C, Molinari F, Romano D (2016) Seawater-based biocatalytic strategy: stereoselective reductions of ketones with marine yeasts. ChemCatChem. https://doi.org/10.1002/cctc.201600947
- Yamamoto K, Oishi K, Fujimatsu I, Komatsu K (1991) Production of R-(−)-Mandelic acid from Mandelonitrile by Alcaligenes faecalis ATCC 8750. Appl Environ Microbiol 57:3028–3032Google Scholar
- Zhang Q, Gong J, Dong T, Liu T, Li H, Dou W, Lu Z, Shi J, Xu Z (2017) Nitrile-hydrolyzing enzyme from Meyerozyma guilliermondii and its potential in biosynthesis of 3-hydroxypropionic acid. Bioprocess Biosyst Eng. https://doi.org/10.1007/s00449-017-1754-6