Intracellular Metabolic Changes of Rhodococcus sp. LH During the Biodegradation of Diesel Oil
In recent years, some marine microbes have been used to degrade diesel oil. However, the exact mechanisms underlying the biodegradation are still poorly understood. In this study, a hypothermophilous marine strain, which can degrade diesel oil in cold seawater was isolated from Antarctic floe-ice and identified and named as Rhodococcus sp. LH. To clarify the biodegradation mechanisms, a gas chromatography-mass spectrometry (GC-MS)-based metabolomics strategy was performed to determine the diesel biodegradation process-associated intracellular biochemical changes in Rhodococcus sp. LH cells. With the aid of partial least squares-discriminant analysis (PLS-DA), 17 differential metabolites with variable importance in the projection (VIP) value greater than 1 were identified. Results indicated that the biodegradation of diesel oil by Rhodococcus sp. LH was affected by many different factors. Rhodococcus sp. LH could degrade diesel oil through terminal or sub-terminal oxidation reactions, and might also possess the ability to degrade aromatic hydrocarbons. In addition, some surfactants, especially fatty acids, which were secreted by Rhodococcus into medium could also assist the strain in dispersing and absorbing diesel oil. Lack of nitrogen in the seawater would lead to nitrogen starvation, thereby restraining the amino acid circulation in Rhodococcus sp. LH. Moreover, nitrogen starvation could also promote the conversation of relative excess carbon source to storage materials, such as 1-monolinoleoylglycerol. These results would provide a comprehensive understanding about the complex mechanisms of diesel oil biodegradation by Rhodococcus sp. LH at the systematic level.
KeywordsDiesel oil biodegradation Rhodococcus sp. LH Molecular mechanisms Metabolomics
Gas chromatography-mass spectrometry
Hierarchical cluster analysis
Principal components analysis
Partial least squares-discriminant analysis
Retention time-mass to charge ratio
Standard error of the mean
Total ion chromatogram
Variable importance in the projection
This work was funded by the Basic Scientific Fund for National Public Research Institutes of China (No. 2016Q10) and the National Natural Science Foundation of China (No. 41776203).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- Auffret MD, Yergeau E, Labbé D, Fayolleguichard F, Greer CW (2015) Importance of Rhodococcus strains in a bacterial consortium degrading a mixture of hydrocarbons, gasoline, and diesel oil additives revealed by metatranscriptomic analysis. Appl Microbiol Biotechnol 99:2419–2430CrossRefPubMedCentralGoogle Scholar
- Li Z, Feiten HJ, Chang D, Duetz WA, van Beilen JB, Witholt B (2001) Preparation of (R)- and (S)-N-protected 3-hydroxypyrrolidines by hydroxylation with Sphingomonas sp. HXN-200, a highly active, regio- and stereoselective, and easy to handle biocatalyst. J Org Chem 66:8424–8430CrossRefPubMedCentralGoogle Scholar