Marine Biotechnology

, Volume 20, Issue 2, pp 246–256 | Cite as

Characterization of Spleen Transcriptome of Schizothorax prenanti during Aeromonas hydrophila Infection

  • Hua Ye
  • Shijun Xiao
  • Xiaoqing Wang
  • Zhiyong Wang
  • Zhengshi Zhang
  • Chengke Zhu
  • Bingjie Hu
  • Changhuan Lv
  • Shuming Zheng
  • Hui Luo
Original Article

Abstract

Schizothorax prenanti (S. prenanti) is an indigenous fish species and is popularly cultured in southwestern China. In recent years, intensive farming of S. prenanti and water quality deterioration has increased the susceptibility of this fish to various pathogens, including Aeromonas hydrophila (A. hydrophila), which has caused severe damage to S. prenanti production. However, the understanding of molecular immune response of S. prenanti to A. hydrophila infection is still lacking. In order to better comprehend the S. prenanti time series immune response process against A. hydrophila, we conducted the first transcriptomic comparison in S. prenanti spleen at 4, 24, and 48 h after the infection challenge of A. hydrophila against their control counterparts. In total, 628 million clean reads were obtained from 18 libraries and assembled into 262,745 transcripts. After eliminating sequence redundancy, 69,373 unigenes with an average length of 1476 bp were obtained. Comparative analysis revealed 1890 unigenes with significantly differential expression, including 172, 455, 589 upregulated and 27, 676, 551 unigenes downregulated genes for 4, 24, and 48 h post-infection, respectively. Differentially expressed genes (DEGs) were validated using qPCR for 15 randomly selected genes. Enrichment and pathway analysis of DEGs was carried out to understand the functions of the immune-related genes. Our results revealed that many important functional genes relating to complement and coagulation cascades, chemokine signaling pathway, toll-like receptor signaling pathway, NOD-like receptor signaling pathway and leukocyte transendothelial migration were regulated during the infection of A. hydrophila, and the expression of those genes reflected the transcriptome profiles during the challenging stages.

Keywords

Schizothorax prenanti Aeromonas hydrophila Transcriptome Immune-related gene Differentially expressed genes 

Notes

Acknowledgments

We thank Lingbing Zeng, in Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences, and Bin Chen, in College of Animal Science and Technology, Hunan Agricultural University, for providing constructive suggestions on the experiments. We also thank Genhua Yue and Baoqing Ye, Molecular Population Genetics Group, Temasek Life Sciences Laboratory, 1 research link, National University of Singapore, for providing constructive suggestions on the manuscript.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Animal ScienceSouthwest UniversityChongqingChina
  2. 2.Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of ChongqingChongqingChina
  3. 3.College of Animal Science and TechnologyHunan Agricultural UniversityChangshaChina
  4. 4.Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan ProvinceChangdeChina
  5. 5.Key Laboratory of Healthy Mariculture for East China Sea of Agriculture Ministry, Fisheries CollegeJimei UniversityXiamenChina

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