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Dynamics of DNA Methylation and DNMT Expression During Gametogenesis and Early Development of Scallop Patinopecten yessoensis

  • Yangping Li
  • Lingling Zhang
  • Yajuan Li
  • Wanru Li
  • Zhenyi Guo
  • Ruojiao Li
  • Xiaoli Hu
  • Zhenmin Bao
  • Shi WangEmail author
Original Article

Abstract

DNA methylation reprograms during gametogenesis and embryo development, which is essential for germ cell specification and genomic imprinting in mammals. Corresponding process remains poorly investigated in molluscs. Here, we examined global DNA methylation level in the gonads of scallop Patinopecten yessoensis during gametogenesis and in embryos/larvae at different stages. DNA methylation level fluctuates during gametogenesis and early development, peaking at proliferative stage of ovary, growing stage of testis, and in blastulae. To understand the mechanisms underlying these changes, we conducted genome-wide characterization of DNMT family and investigated their expression profiles based on transcriptomes and in situ hybridization. Three genes were identified, namely PyDNMT1, PyDNMT2, and PyDNMT3. Expression of PyDnmt3 agrees with DNA methylation level during oogenesis and early development, suggesting PyDNMT3 may participate in de novo DNA methylation that occurs mainly at proliferative stage of ovary and testis, and in blastulae and gastrulae. PyDnmt1 expression is positively correlated with DNA methylation level during spermatogenesis, and is higher at maturation stage of ovary and in 2–8 cell embryos than other stages, implying possible involvement of PyDNMT1 in DNA methylation maintenance during meiosis and embryonic development. This study will facilitate better understanding of the developmental epigenetic reprogramming in bivalve molluscs.

Keywords

DNA methylation DNMT Gametogenesis Early development Scallop 

Notes

Funding Information

This work was supported by the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) (2018SDKJ0302-1), National Natural Science Foundation of China (31871499 and 31572600), Major basic research projects of Shandong Natural Science Foundation (ZR2018ZA0748) and Fundamental Research Funds for the Central Universities (201762001).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

10126_2018_9871_MOESM1_ESM.pdf (2.3 mb)
Fig. S1 Alignment of the DNA methylase (DM) domains of DNMT1 (A), DNMT2 (B) and DNMT3 (C) from different organisms. (PDF 2405 kb)
10126_2018_9871_Fig7_ESM.png (89 kb)
Fig. S2

Minisatellite and the predicted protein sequences in the DM domain of PyDnmt2. (PNG 88 kb)

10126_2018_9871_MOESM2_ESM.tif (47 kb)
High resolution image (TIF 46 kb)

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.MOE Key Laboratory of Marine Genetics and BreedingOcean University of ChinaQingdaoChina
  2. 2.Laboratory for Marine Fisheries Science and Food Production ProcessesQingdao National Laboratory for Marine Science and TechnologyQingdaoChina
  3. 3.Laboratory for Marine Biology and BiotechnologyQingdao National Laboratory for Marine Science and TechnologyQingdaoChina

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