Wheat storage proteins: changes on the glutenins after wheat infection with different isolates of Fusarium graminearum

  • Leonel Maximiliano Ortega
  • María Candela Moure
  • Esteban Manuel González
  • Teresa María AlconadaEmail author
Original Article


Wheat gluten proteins are decisive for the industrial properties of flour, so alterations resulting from grain infection with Fusarium graminearum produce changes in the glutenin content that affect the baking properties. This work analyzes the high-molecular-weight glutenin changes from wheat flour with different degrees of F. graminearum infection at field, since these proteins are determinant for the quality properties of flour. Wheat cultivars—on field trials—infected with F. graminearum isolates of diverse aggressiveness showed severity values between 9.1 and 42.58% and thousand kernel weight values between 28.12 and 32.33 g. Negative correlations between severity and protein content and positive correlations between yield and protein content were observed, employing reversed-phase high-performance liquid chromatography and polyacrylamide gel electrophoresis. Furthermore, the protein signal changes were in agreement for both methodological approaches. Also, the degree of disease observed and the protein changes on infected wheat cultivars varied in relation with the aggressiveness of the isolate responsible for the infection. The principal component analysis showed a close arrangement among protein values obtained by HPLC. For each cultivar, two principal components were obtained, which explained 80.85%, 88.48%, and 93.33% of the total variance (cultivars Sy200, AGP Fast, and Klein Tigre respectively). To our knowledge, the approaches employed for the analysis of protein changes according to the degree of disease, as well as the thorough statistical analysis, are novel for the study of Fusarium Head Blight.


Whole grain flour Aggressiveness Fungal infection Gluten proteins HPLC analysis SDS-PAGE analysis 


Funding information

We thank Consejo Nacional de Investigaciones Científicas y Tecnológica (Grant PIP 0819) for the financial support.

Compliance with ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.


  1. Akinsanmi OA, Backhouse DA, Simpfendorfer SR, Chakraborty S (2008) Mycelial compatibility reactions of Australian Fusarium graminearum and F. pseudograminearum isolates compared with AFLP groupings. Plant Pathol 57:251–261CrossRefGoogle Scholar
  2. Alberione EJ, Ortega LM, Salines N, Astoreca AL, Alconada TM (2016) Genetic behavior of different wheat genotypes against Fusarium graminearum. Agrociencia 250:335–346Google Scholar
  3. Alvarez CL, Somma S, Proctor RH, Stea G, Mulè G, Logrieco AFR, Fernández Pinto VE, Moretti A (2011) Genetic diversity in Fusarium graminearum from a major wheat-producing region of Argentina. Toxins 3:1294–1309CrossRefGoogle Scholar
  4. Barneix AJ (2007) Physiology and biochemistry of source-regulated protein accumulation in the wheat grain. J Plant Physiol 164:581–590CrossRefGoogle Scholar
  5. Belton PS (2005) New approaches to study the molecular basis of the mechanical properties of gluten. J Cereal Sci 41:203–211CrossRefGoogle Scholar
  6. Brzozowski B, Dawidziuk K, Bednarski W (2008) Gliadin degradation by proteases of Fusarium genus fungi in different in vivo and in vitro conditions. Pol J Nat Sci 23:188–206CrossRefGoogle Scholar
  7. Castañares E, Albuquerque DR, Dinolfo MI, Fernández Pinto VE, Patriarca A, Stenglein SA (2014) Trichothecene genotypes and production profiles of Fusarium graminearum isolates obtained from barley cultivated in Argentina. Int J Food Microbiol 179:57–63CrossRefGoogle Scholar
  8. D’Ovidio R, Masci S (2004) The low-molecular-weight glutenin subunits of wheat gluten. J Cereal Sci 39:321–339CrossRefGoogle Scholar
  9. DuPont FM, Altenbach SB (2003) Molecular and biochemical impacts of environmental factors on wheat grain development and protein synthesis. J Cereal Sci 38:133–146CrossRefGoogle Scholar
  10. Eggert K, Wieser H, Pawelzik E (2010) The influence of Fusarium infection and growing location on the quantitative protein composition of (part I) emmer (Triticum dicoccum). Eur Food Res Technol 230:837–847CrossRefGoogle Scholar
  11. Eggert K, Rawel HM, Pawelzik E (2011) In vitro degradation of wheat gluten fractions by Fusarium graminearum proteases. Eur Food Res Technol 233:697–705CrossRefGoogle Scholar
  12. FAO (2015). Worldwide regulations for mycotoxins in food and feed Accessed 3 August 2017
  13. Gianibelli MC, Larroque OR, MacRitchie F, Wrigley CW (2001) Biochemical, genetic, and molecular characterization of wheat glutenin and its component subunits. Cereal Chem 78:635–646CrossRefGoogle Scholar
  14. Gras PW, Anderssen RS, Keentok M, Békés F, Appels R (2001) Gluten protein functionality in wheat flour processing: a review. Aust J Agric Res 52:1311–1323CrossRefGoogle Scholar
  15. Horvat DI, Spanic V, Dvojkovic K, Simic GH, Magdic DN, Nevistic A (2014) The influence of Fusarium infection on wheat (Triticum aestivum L.) proteins distribution and baking quality. Cereal Res Commun 43:61–71CrossRefGoogle Scholar
  16. Kamal AHM, Kim KH, Shin KH, Seo HS, Tsujimoto H, Heo HY, Choi JS, Park CS, Woo SH (2009) Diversity of novel glutenin subunits in bread wheat (Triticum aestivum L.). J Plant Biol 52:533–542CrossRefGoogle Scholar
  17. Kang Z, Buchenauer H (2000) Ultrastructural and cytochemical studies on cellulose, xylan and pectin degradation in wheat spikes infected by Fusarium culmorum. J Phytopathol 148:263–275CrossRefGoogle Scholar
  18. Kang Z, Zingen-Sell I, Buchenauer H (2005) Infection of wheat spikes by Fusarium avenaceum and alterations of cell wall components in the infected tissue. Eur J Plant Pathol 111:19–28CrossRefGoogle Scholar
  19. Kikot GE, Hours RA, Alconada TM (2009) Contribution of cell wall degrading enzymes to pathogenesis of Fusarium graminearum: a review. J Basic Microbiol 49:231–241CrossRefGoogle Scholar
  20. Kikot GE, Moschini RC, Consolo VF, Rojo RA, Salerno GL, Hours RA, Gasoni LA, Arambarri AM, Alconada Magliano TM (2011) Occurrence of different species of Fusarium from wheat in relation to disease levels predicted by a weather-based model in Argentina Pampas region. Mycopathologia 117:139–149CrossRefGoogle Scholar
  21. Kreuzberger M, Limsuwan S, Eggert K, Karlovsky P, Pawelzik E (2015) Impact of Fusarium spp. infection of bread wheat (Triticum aestivum L.) on composition and quality of flour in association with EU maximum level for deoxynivalenol. J Appl Bot Food Qual 88:177–185Google Scholar
  22. Larroque OR, Gianibelli MC, Gomez Sanchez M, MacRitchie F (2000) Procedure for obtaining stable protein extracts of cereal flour and whole meal for size-exclusion HPLC analysis. Cereal Chem 77:448–450CrossRefGoogle Scholar
  23. Malbrán I, Mourelos CA, Girotti JR, Aulicino MB, Balatti PA, Lori GA (2012) Aggressiveness variation of Fusarium graminearum isolates from Argentina following point inoculation of field grown wheat spikes. Crop Prot 42:234–243CrossRefGoogle Scholar
  24. McMullen MP, Jones RK, Gallemberg D (1997) Scab of wheat and barley: a re-emerging disease of devastating impact. Plant Dis 81:1340–1348CrossRefGoogle Scholar
  25. Mesterházy Á, Bartók T, Mirocha CG, Komoróczy R (1999) Nature of wheat resistance to Fusarium head blight and the role of deoxynivalenol for breeding. Plant Breed 118:97–110CrossRefGoogle Scholar
  26. Monds RD, Cromey MG, Lauren DR, di Menna M, Marshall J (2005) Fusarium graminearum, F. cortaderieae and F. pseudograminearum in New Zealand: molecular phylogenetic analysis,mycotoxin chemotypes and coexistence of species. Mycol Res 109:410–420CrossRefGoogle Scholar
  27. Naeem HA, Sapirstein HD (2007) Ultra-fast separation of wheat glutenin subunits by reversed-phase HPLC using a superficially porous silica-based column. J Cereal Sci 46:157–168CrossRefGoogle Scholar
  28. Nightingale MJ, Marchylo BA, Clear RM, Dexter JE, Preston KR (1999) Fusarium head blight: effect of fungal proteases on wheat storage proteins. Cereal Chem 76(1):150–158CrossRefGoogle Scholar
  29. Ortega LM (2017) Caracterización de aislamientos de Fusarium graminearum y su relación con el deterioro de granos de trigo infectados. National University of La Plata Argentina, Doctoral dissertationGoogle Scholar
  30. Ortega LM, Dinolfo MI, Astoreca AL, Alberione EJ, Stenglein SA, Alconada TM (2016) Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina. Mycol Prog 15(1):1–8CrossRefGoogle Scholar
  31. Ortega LM, Moure MC, Astoreca AL, Alberione EJ, Alconada TM (2018) Wheat grains damaged by Fusarium graminearum: alterations in yield, toxicity and protein composition. Vegetos 31:2Google Scholar
  32. Palacios SA, Ramírez ML, Cabrera Zalazar M, Farnochi MC, Zappacosta D, Chiacchiera SM, Reynoso MM, Chulze SN, Torres AM (2011) Occurrence of Fusarium spp. and fumonisin in durum wheat grains. J Agric Food Chem 59:12264–12,269CrossRefGoogle Scholar
  33. Payne PI, Nightingale MA, Krattiger AF, Holt LM (1987) The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties. J Sci Food Agric 40:51–65CrossRefGoogle Scholar
  34. Pritsch C, Muehlbauer GJ, Bushnell WR, Somers DA, Vance CP (2000) Fungal development and induction of defense response genes during early infection of wheat spikes by Fusarium graminearum. Mol Plant-Microbe Interact 13:159–169CrossRefGoogle Scholar
  35. Shewry PR, Hawkesford MJ, Piironen V, Lampi AM, Gebruers K, Boros D, Andersson AAM, Aman P, Rakszegi M, Bedo Z, Ward JL (2013) Natural variation in grain composition of wheat and related cereals. J Agric Food Chem 61:8295–8303CrossRefGoogle Scholar
  36. Tóth B, Mesterházy Á, Horváth Z, Bartók T, Varga M, Varga J (2005) Genetic variability of central European isolates of the Fusarium graminearum species complex. Eur J Plant Pathol 113:35–45CrossRefGoogle Scholar
  37. Ueno T, Stevenson SG, Preston KR, Nightingale MJ, Marchylo BM (2002) Simplified dilute acetic acid based extraction procedure for fractionation and analysis of wheat flour protein by size exclusion HPLC and flow field-flow fractionation. Cereal Chem 79:155–161CrossRefGoogle Scholar
  38. Wang J, Wieser H, Pawelzik E, Weinert J, Keutgen AJ, Wolf GA (2005) Impact of the fungal protease produced by Fusarium culmorum on the protein quality and breadmaking properties of winter wheat. Eur Food Res Technol 220:552–559CrossRefGoogle Scholar
  39. Wanjiru WM, Zhensheng K, Buchenauer H (2002) Importance of cell wall degrading enzymes produced by Fusarium graminearum during infection of wheat heads. Eur J Plant Pathol 108:803–810CrossRefGoogle Scholar
  40. Wieser H (2007) Chemistry of gluten proteins. Food Microbiol 24:115–119CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI), UNLP, CCT-La Plata, CONICET, Facultad de Ciencias ExactasUniversidad Nacional de La PlataLa PlataArgentina

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