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International Microbiology

, Volume 22, Issue 1, pp 143–154 | Cite as

An Insight into the Constitutive Proteome Throughout Leishmania donovani Promastigote Growth and Differentiation

  • Pedro J. AlcoleaEmail author
  • Ana Alonso
  • Francisco García-Tabares
  • María del Carmen Mena
  • Sergio Ciordia
  • Vicente Larraga
Original Article

Abstract

Anthroponotic visceral leishmaniasis is a life-threatening disease caused by Leishmania donovani (Kinetoplastida: Trypanosomatidae) in East Africa and the Indian subcontinent. Unlike promastigote growth and differentiation in the sand fly gut or in axenic culture, L. donovani promastigote-into-amastigote development has been studied by high-throughput gene expression profiling. In this study, we have identified abundant constitutive proteins in axenically cultured promastigotes by two-dimension electrophoresis and matrix-assisted laser desorption-ionization tandem time-of-flight (MALDI-TOF/TOF) mass spectrometry. Most proteins involved in the trypanothione-based redox antioxidant system are expressed constitutively throughout axenic L. donovani promastigote growth and differentiation (tryparedoxin, trypanothione peroxidase, generic peroxidoxin, iron-superoxide dismutase, and elongation factor 1β). These findings are in agreement with previous data on other Old World species (i.e., L. major and L. infantum), whereas New World species (i.e., L. amazonensis and L. pifanoi) and Crithidia fasciculata show different expression patterns.

Keywords

Leishmania donovani Constitutive proteins 2DE MALDI-TOF/TOF Elongation factor 1β Peroxiredoxins Tryparedoxin peroxidase Iron superoxide dismutase 

Notes

Acknowledgments

We acknowledge Alfredo Toraño and Mercedes Domínguez for kind supply of the L. donovani strain used in this study. The CIB-CSIC Laboratory of Molecular Parasitology thanks the Ramón Areces Foundation (2016 call) for a contract. The CNB-CSIC Proteomics Facility belongs to ProteoRed (PRB2-ISCIII) and has been funded with grant PT13/0001.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest

Supplementary material

10123_2018_36_MOESM1_ESM.xlsx (19 kb)
ESM 1 (XLSX 18 kb)

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Laboratory of Molecular Parasitology, Department of Cellular and Molecular BiologyCentro de Investigaciones Biológicas (Consejo Superior de Investigaciones Científicas)MadridSpain
  2. 2.Service of Proteomics and GenomicsCentro de Investigaciones Biológicas (Consejo Superior de Investigaciones Científicas)MadridSpain
  3. 3.Proteomics UnitCentro Nacional de Biotecnología (Consejo Superior de Investigaciones Científicas)MadridSpain

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