Gastric Cancer

, Volume 22, Issue 4, pp 705–722 | Cite as

MiR-6872 host gene SEMA3B and its antisense lncRNA SEMA3B-AS1 function synergistically to suppress gastric cardia adenocarcinoma progression

  • Wei Guo
  • Xiaoliang Liang
  • Lei Liu
  • Yanli Guo
  • Supeng Shen
  • Jia Liang
  • Zhiming DongEmail author
Original Article



Semaphorin 3B (SEMA3B) is frequently inactivated in several carcinomas. However, as the host gene of miR-6872, the roles of SEMA3B, antisense lncRNA SEMA3B-AS1, and miR-6872 in gastric cardia adenocarcinoma (GCA) tumorigenesis have not been clarified.


The expression levels of SEMA3B, SEMA3B-AS1, and miR-6872 were respectively detected by qRT-PCR, western blot, or immunohistochemical staining assays. The methylation status was determined by BGS and BS-MSP methods. In vitro assays were preformed to explore the biological effects of SEMA3B, SEMA3B-AS1, and miR-6872-5p in gastric cancer cells. Chromatin immunoprecipitation assay was used to detect the binding of protein to DNA. The interaction of SEMA3B-AS1 with MLL4 was identified by RNA immunoprecipitation and RNA pull-down assays.


Frequent downregulation of SEMA3B, SEMA3B-AS1, and miR-6872 was detected in GCA tissues and gastric cancer cells. Aberrant hypermethylation of the promoter region was more tumor specific and was negatively correlated with the expression level of SEMA3B, SEMA3B-AS1, and miR-6872-5p. Transcription factor Sp1 activated SEMA3B or SEMA3B-AS1 transcription and CpG sites hypermethylation within promoter region eliminated Sp1 binding ability. Overexpression of SEMA3B and SEMA3B-AS1 inhibited gastric cancer cell proliferation, migration, and invasion in vitro. SEMA3B-AS1 induced the expression of SEMA3B by interacting with MLL4. ZNF143 might be the target gene of miR-6872-5p and miR-6872-5p functioning synergistically with SEMA3B to suppress cell invasion. Furthermore, SEMA3B, SEMA3B-AS1, and miR-6872-5p expression levels were associated with GCA patients’ survival.


SEMA3B, SEMA3B-AS1, and miR-6872 may act as tumor suppressors and may serve as potential targets for antitumor therapy.


SEMA3B SEMA3B-AS1 MiR-6872 Gastric cardia adenocarcinoma Expression 



This study was supported by Grants from the National Natural Science Foundation (Nos. 81472335, 81572441, 81772612), Natural Science Foundation of Hebei Province (Nos. H2015206196 and H2015206420).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

The study was approved by the Ethics Committee of the Fourth Hospital, Hebei Medical University.

Informed consent

Informed consent was obtained from all participants included in the study.

Supplementary material

10120_2019_924_MOESM1_ESM.docx (1 mb)
Supplementary material 1 (DOCX 1036 KB)


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Copyright information

© The International Gastric Cancer Association and The Japanese Gastric Cancer Association 2019

Authors and Affiliations

  • Wei Guo
    • 1
  • Xiaoliang Liang
    • 1
  • Lei Liu
    • 2
  • Yanli Guo
    • 1
  • Supeng Shen
    • 1
  • Jia Liang
    • 1
  • Zhiming Dong
    • 1
    Email author
  1. 1.Laboratory of Pathology, Hebei Cancer InstituteThe Fourth Hospital of Hebei Medical UniversityShijiazhuangChina
  2. 2.Department of Thoracic SurgeryThe Fourth Hospital of Hebei Medical UniversityShijiazhuangChina

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