miR-548b-3p functions as a tumor suppressor in lung cancer
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miR-548-3p is one of the members of miR-548 family, a large primate-specific miRNA gene family. The role of miR-548-3p in lung cancer was less studied. In this study, we found the expression of miR-548-3p was lower both in clinical tumor specimens and lung cancer cells compared with normal controls. In vitro, miR-548-3p inhibited lung cancer cell growth and promoted cell apoptosis at the S stage of cell cycle. The underlying mechanism of miR-548b-3p-induced cell proliferation inhibition and apoptosis may be associated with the inhibition of PI3K/AKT signaling pathway. In vivo, miR-548b-3p also suppressed tumor growth in xenografts model of lung cancer cells. Our results indicated that miR-548b-3p might be an anti-tumor target of lung cancer in the future.
KeywordsLung cancer miR-548b-3p PI3K/AKT signaling pathway Tumor suppressor
Z.l.W, X.f.W. X.w.H. W.q.Z., and C.Y. conducted the experiments and provided experimental methods and data. Z.l.W, W.W., and L.x.C. designed the study, analyzed the data, and wrote the manuscript.
This work was supported by Shanghai Municipal Planning Commission of Science and Research (201840209).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All experimental protocols and methods were approved by the XuZhou City Hospital of Medical College of TCM, No 20190422. We also confirmed that all methods were performed in accordance with the relevant guidelines and regulations. Mice were bred in the Animal Core Facility by following the procedures approved by the XuZhou City Hospital of Medical College of Institutional Animal Care and Use Committee.
- 1.Ferlay J, Colombet M, Soerjomataram I, Mathers C, Parkin DM, Pineros M, Znaor A, Bray F (2019) Estimating the global cancer incidence and mortality in 2018: GLOBOCAN sources and methods. Int J Cancer 144:1941–1953Google Scholar
- 3.Wu KL, Tsai YM, Lien CT, Kuo PL, Hung AJ (2019) The Roles of MicroRNA in Lung Cancer. Int J Mol Sci 20, 1–25Google Scholar
- 7.Jin Z, Guan L, Song Y, Xiang GM, Chen SX, Gao B (2016) MicroRNA-138 regulates chemoresistance in human non-small cell lung cancer via epithelial mesenchymal transition. Eur Rev Med Pharmacol Sci 20:1080–1086Google Scholar
- 11.Arechaga-Ocampo E, Lopez-Camarillo C, Villegas-Sepulveda N, Gonzalez-De la Rosa CH, Perez-Añorve IX, Roldan-Perez R, Flores-Perez A, Peña-Curiel O, Angeles-Zaragoza O, Rangel Corona R, Gonzalez-Barrios JA, Bonilla-Moreno R, Del Moral-Hernandez O, Herrera LA, Garcia-Carranca A (2017) Tumor suppressor miR-29c regulates radioresistance in lung cancer cells. Tumour Biol 39, 1–14Google Scholar
- 20.Aziz, A.U.R., Farid, S., Qin, K., Wang, H. and Liu, B. (2018). PIM Kinases and Their Relevance to the PI3K/AKT/mTOR Pathway in the Regulation ofOvarian Cancer. Biomolecules 8, 1–14Google Scholar
- 21.Shen, W.M., Yin, J.N., Xu, R.J., Xu, D.F. and Zheng, S.Y. (2019). Ubiquitin specific peptidase 49 inhibits non-small cell lung cancer cell growth bysuppressing PI3K/AKT signaling. Kaohsiung J Med Sci 35. 401–407Google Scholar
- 22.Guo C, Wang L, Zhao Y, Jiang B, Luo J, Shi D (2019) BOS-93, a novel bromophenol derivative, induces apoptosis and autophagy in human A549 lung cancer cells via PI3K/Akt/mTOR and MAPK signaling pathway. Exp Ther Med 17:3848–3858Google Scholar
- 25.Saffari, M., Ghaderian, S.M.H., Omrani, M.D., Afsharpad, M., Shankaie, K. and Samadaian, N. (2018). The Association of miR-let 7b and miR-548 with PTEN in Prostate Cancer. Urol J 16, 267–273Google Scholar